Pichia pastoris composition expressed aerolysin mutant of Aeromonas veronii as an oral vaccine evaluated in zebrafish (Danio rerio)

文献类型： 外文期刊

第一作者： Yao, Yuan-Yuan

作者： Yao, Yuan-Yuan;Yang, Ya-Lin;Ran, Chao;Zhang, Zhen;Zhang, Qing-Shuang;Liu, Shu-Bin;Yang, Hong-Wei;Chen, Xing-Yu;Gao, Chen-Chen;Teame, Tsegay;Zhou, Zhi-Gang;Yang, Hong-Wei;Teame, Tsegay

作者机构：

关键词： Aeromonas veronii; Aerolysin mutant NTaer; Oral vaccine; Immune response; Gut microbiota

期刊名称：MARINE LIFE SCIENCE & TECHNOLOGY （ 影响因子：5.8； 五年影响因子：5.7 ）

ISSN： 2096-6490

年卷期： 2024 年 6 卷 3 期

页码：

收录情况： SCI

摘要： Vaccines are one of the most practical means to stop the spreading of Aeromonas veronii in aquaculture. In this study, virulence factor aerolysin mutant NTaer which has lost its hemolytic activity was used as a target antigen. Pichia pastoris constitutive secretory expression NTaer (GS115-NTaer) was used as a potential safe oral vaccine to evaluate its effectiveness on zebrafish immunity. The result shows that vaccination of GS115- NTaer for four weeks did not affect the growth performance of the host, while eliciting an effective immune protective response. Compared with the control group, the GS115-NTaer could significantly up-regulate the relative expression level of the intestinal tight junction protein 1 alpha (TJP1 alpha) gene, and significantly increased the contents of lysozyme (LYZ), complement C3 and C4 in the gut, indicating that the innate immune response of the fish was activated. The relative gene expression levels of macrophage-expressed gene 1 (MPEG1) and T cell receptor (TCR-alpha) in the gut, and MPEG1, CD4, CD8, TCR-alpha, GATA3, and T-bet in the spleen were all increased significantly, indicating that the cellular immune response of the fish was activated. Furthermore, the contents of serum IgM and intestinal mucosa IgZ antibodies were significantly increased, which showed that humoral immunity was also activated. Moreover, inoculation with GS115-NTaer significantly changed the structure of gut microbiota. In particular, the relative ratio of (Firmicutes + Fusobacteriota + Bacteroidota)/Proteobacteria was significantly higher than that of the control and GS115 groups. Lastly, the vaccinated fish were challenged with A. veronii, and the relative percent survival of GS115 and the GS115-NTear groups was 14.28% and 33.43%. This improvement of immunity was not only due to the specific immune response but also attributed to the improvement of innate immunity and the gut microbiota which was demonstrated by the germ-free zebrafish model. Collectively, this study provides information on the effectiveness of GS115-NTear as an oral vaccine for the green prevention and control of A. veronii infection in fish aquaculture.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序