Isolation functional characterization of allatotropin receptor from the cotton bollworm, Helicoverpa armigera

文献类型： 外文期刊

第一作者： Zhang, Fang

作者： Zhang, Fang;Wang, Jun;Thakur, Kiran;Hu, Fei;Zhang, Jian-Guo;Jiang, Li;Wei, Zhao-Jun;Jiang, Xing-Fu;An, Shi-Hen;Jiang, Hongbo

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关键词： Allatotropin; G protein-coupled receptors; Neuropeptides; Expression; Helicoverpa armigera

期刊名称：PEPTIDES （ 影响因子：3.75； 五年影响因子：3.389 ）

ISSN： 0196-9781

年卷期： 2019 年 122 卷

页码：

收录情况： SCI

摘要： Insect allatotropin (AT) plays multi-functions including regulation of juvenile hormone synthesis, growth, development and reproduction. In the present study, the full-length cDNA encoding the AT receptor was cloned from the brain of Helicoverpa armigera (Helar-ATR). The ORF of Helar-ATR exhibited the characteristic seven transmembrane domains of the G protein-coupled receptor (GPCR) and was close to the ATR of Manduca sexta in the phylogenetic tree. The Helar-ATR expressed in vertebrate cell lines can be activated by Helar-AT and each Helar-ATL in a dose-responsive manner, in the following order: Helar-ATLI > Helar-ATLII > Helar-AT > Helar-ATLIII. Helar-ATLI and Helar-ATLII represented the functional ligands to Helar-ATR in vitro, while Helar-AT and Helar-ATLIII behaved as partial agonists. The in vitro functional analysis suggested that the Helar-ATR signal was mainly coupled with elevated levels of Ca2+ and independent of cAMP levels. Helar-ATR mRNA in larvae showed the highest level in the brain, followed by the thorax ganglion, abdomen ganglion, fat body and midgut. Helar-ATR mRNA levels in the complex of the brain-thoracic-abdomen ganglion on the 2nd day of the larval stage and during later pupal stages were observed to be relatively higher than in the wandering and early pupal stages.

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