A novel cascade catalysis for one-pot enzymatically modified isoquercitrin (EMIQ) conversion from rutin and sucrose using rationally designed gradient temperature control

文献类型： 外文期刊

第一作者： Zhan, Yu-Fan

作者： Zhan, Yu-Fan;Meng, Zhuo-Hao;Yan, Cheng-Hai;Tan, Min;Khurshid, Marriam;Li, Yi-Jiangcheng;Zheng, Shao-Jun;Wang, Jun;Zhan, Yu-Fan;Meng, Zhuo-Hao;Yan, Cheng-Hai;Tan, Min;Khurshid, Marriam;Li, Yi-Jiangcheng;Zheng, Shao-Jun;Wang, Jun;Wang, Jun

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关键词： Cascade reaction; alpha-L-rhamnosidase; Deinococcus geothermalis; Amylosucrase; Enzymatically modified isoquercitrin.

期刊名称：FOOD CHEMISTRY （ 影响因子：8.5； 五年影响因子：8.2 ）

ISSN： 0308-8146

年卷期： 2024 年 457 卷

页码：

收录情况： SCI

摘要： Enzymatically modified isoquercitrin (EMIQ) is a glyco-chemically modified flavonoid exhibiting notably high biological activity, such as antioxidant, anti-inflammatory and anti-allergic properties. However, the utilization of expensive substrates such as isoquercitrin and cyclodextrin in the conventional approach has hindered the industrial-scale production of EMIQ due to high cost and low yields. Hence, the development of a cost-effective and efficient method is crucial for the biological synthesis of EMIQ. In this study, a natural cascade catalytic reaction system was constructed with alpha-L-rhamnosidase and amylosucrase using the inexpensive substrates rutin and sucrose. Additionally, a novel approach integrating gradient temperature regulation into biological cascade reactions was implemented. Under the optimal conditions, the rutin conversion reached a remarkable 95.39% at 24 h. Meanwhile, the productivity of quercetin-3-O-tetraglucoside with the best bioavailability reached an impressive 41.69%. This study presents promising prospects for future mass production of EMIQ directly prepared from rutin and sucrose.

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