文献类型： 外文期刊
第一作者： Chen, M.
作者： Chen, M.;Ye, N.;Liu, Y.;An, J.
作者机构：
关键词： Bumblebees; PGRP-LC; phylogenetic tree; purification
期刊名称：SOCIOBIOLOGY （ 影响因子：0.983； 五年影响因子：1.01 ）
ISSN： 0361-6525
年卷期： 2019 年 66 卷 2 期
页码：
收录情况： SCI
摘要： Peptidoglycan recognize protein LC (PGRP-LC) is a significant pattern recognition receptor of the insect innate immune system that can recognize peptidoglycans and activate immune signaling pathways regulating the expression and release of antimicrobial peptides against infection. We for the first time analyzed the phylogenetic tree, purification and structure of bumblebee PGRP-LC. The results showed high conservation of bumblebee PGRP-LC among the 16 bumblebee species, and further phylogenetic analysis showed that the PGRP-LC phylogeny of different subgenera (Subterraneobombus, Megabombus, Melanobombus, Bombus) is consistent with that of the COI gene. Additionally, the phylogeny of PGRP-LCs among Bombus, Apis and the solitary bee Megachile rotundata coincides with the sociality evolution of bees. Moreover, bumblebee PGRP-LC (BI-PGRP-LC) shares the Drosophila PGRP-LCx and PGRP-LCa topology, retaining conserved disulfide bonds and 80% binding residues involved in the interaction between tracheal cytotoxin (TCT) and PGRP-LCx. Therefore, BI-PGRP-LC might share some similar binding characteristics with Drosophila PGRP-LCx. In addition, BI-PGRP-LC has shorter beta 5 and beta 1 sheets, longer beta 2, beta 3, and beta 4 sheets and a shallow binding groove. To determine the characteristics of BI-PGRP-LC, high-purity PGRP-LC inclusion bodies, soluble GST-tag BI-PGRP-LC fusion protein and soluble pure BI-PGRP-LC were obtained in vitro. The results will be helpful for further study of the function and structure of BI-PGRP-LC.
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