Development and application of an indirect ELISA for the serological detection of duck Tembusu virus infection based on the NS1 protein antigen
文献类型： 外文期刊
第一作者： Zhou, Qi
作者： Zhou, Qi;Bi, Zhuangli;Yin, Dongdong;Gu, Xiangxue;Xu, Zejun;Huang, Rong;Xing, Xue;Wang, Guijun;Bi, Zhuangli;Yin, Dongdong;Qi, Kezong;Wang, Guijun
作者机构：
期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.574； 五年影响因子：2.466 ）
ISSN： 0304-8608
年卷期： 2020 年 165 卷 3 期
页码：
收录情况： SCI
摘要： Duck Tembusu virus (DTMUV) has caused significant economic losses in China since 2010. However, there is still a lack of effective methods to diagnose the disease caused by this virus, and especially to differentiate infection from vaccination. In this study, we established a novel indirect enzyme-linked immunosorbent assay (iELISA) and performed a retrospective serological survey for DTMUV in Anhui province, China. Our results show that the iELISA displayed high specificity sensitivity, and with no serological cross-reaction with other duck pathogens. These findings indicate that the newly developed iELISA could be a useful screening tool for large-scale monitoring of the epidemiology of DTMUV infection in ducks.
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