Identification of a linear B-cell epitope on glycoprotein (GP) 2a of porcine reproductive and respiratory syndrome virus (PRRSV)

文献类型： 外文期刊

第一作者： Shi, Xibao

作者： Shi, Xibao;Fan, Xiaomin;Zhang, Xiaozhuan;Ji, Shengdong;Nie, Shouyi;Kou, Liyun;Liu, Hongliang;Wang, Aiping;Zhang, Gaiping;Zhang, Gaiping;Deng, Ruiguang;Zhang, Gaiping

作者机构：

关键词： PRRSV; GP2a; Epitope

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）

ISSN： 0141-8130

年卷期： 2019 年 139 卷

页码：

收录情况： SCI

摘要： Glycoprotein (GP) 2a was a minor structural protein of porcine reproductive and respiratory syndrome virus (PRRSV) and was one of crucial proteins for PRRSV to bind cell receptor, which indicated that there were neutralizing epitopes on GP2a. In the present work, we used mouse anti-GP2a(41-208aa) serum and one GP2a(41-208aa) specific monoclonal antibody (McAb) to identify B-cell epitopes of GP2a by peptide-based ELISA. A liner B-cell epitope F(194)PTPGSRPKLHDFQQ(208) was identified. However, the results of virus neutralization experiment showed that the McAb could not reduce the titers of PRRSV, which indicated that the identified epitope was not the neutralizing epitope of PRRSV. While the amino acid sequence of this epitope was conserved in North American (type 2) PRRSV, which suggested that this epitope might be diagnostic potential for type 2 PRRSV strains. In conclusion, our present work identified a new epitope on GP2a and this epitope might be diagnostic potential for type 2 PRRSV strains. (c) 2019 Published by Elsevier B.V.

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