Efficient promoter editing of the SBEIIb gene enables fine-tuning of the resistant starch content in rice

文献类型： 外文期刊

第一作者： Sang, Shifei

作者： Sang, Shifei;Sun, Xiaohan;Ma, Tengyun;Zhang, Yijing;Yao, Guoqin;Wang, Xinyu;Feng, Liuchun;Li, Junhua;Ji, Shengdong;Cheng, Hongtao;Tan, Xiaoyu

作者机构：

关键词： Functional rice; SBEIIb; Gene editing; Resistant starch

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）

ISSN： 0141-8130

年卷期： 2025 年 290 卷

页码：

收录情况： SCI

摘要： Rice, a staple in diets, undergoes digestion post-consumption, often triggering a swift surge in blood sugar among diabetics, intensifying their health burden. Notably, resistant starch (RS) emerges as a potent ally in fostering satiety and mitigating metabolic syndrome in diabetes. The SBEIIb gene, a key orchestrator of starch branching enzymes, plays a pivotal role in starch synthesis, and its genetic alteration can dramatically boost RS content in rice. Cultivating RS-rich functional germplasms is of great significance for improving human nutrition and health. In this study, the application of CRISPR/Cas9 technology was investigated for precise multi-target editing within the SBEIIb promoter, aiming to generate valuable germplasm resources enriched with RS. The results revealed extensive mutations in the SBEIIb promoter region, resulting in a varied reduction in SBEIIb expression levels. Remarkably, the grains from the homozygous T1 mutant lines displayed a substantial elevation in RS content, ranging from 3 to 12 times greater than that of the wild-type, accompanied by notable alterations in the starch granule morphology of these grains. This study presents an effective breeding strategy for the precise enhancement of RS content and establishes a foundation for further insights into the molecular mechanisms governing cis-element regulation of RS synthesis.

分类号：

  • 相关文献
作者其他论文 更多>>
  • Epigenetics in the modern era of crop improvements

    作者：Xue, Yan;Deng, Xing Wang;Cao, Xiaofeng;Deng, Xian;Cao, Xiaofeng;Deng, Xian;Chen, Xiangsong;Yang, Hongchun;Ding, Yong;Dong, Aiwu;Liu, Bing;Ren, Guodong;Zhang, Yijing;Duan, Cheng-Guo;Fang, Xiaofeng;Qi, Yijun;Gong, Lei;Liu, Bao;Xu, Zheng-Yi;Gong, Zhizhong;Gong, Zhizhong;Gu, Xiaofeng;He, Chongsheng;He, Hang;He, Shengbo;He, Xin-Jian;He, Yan;Tian, Zhixi;He, Yuehui;Jia, Guifang;Wang, Yuan;Jiang, Danhua;Song, Xianwei;Jiang, Jianjun;Lai, Jinsheng;Lai, Jinsheng;Lai, Jinsheng;Lai, Jinsheng;Lai, Jinsheng;Lang, Zhaobo;Zhu, Jian-Kang;Li, Chenlong;Li, Qing;Li, Xingwang;Yan, Wenhao;Zhou, Dao-Xiu;Luo, Xiao;Qian, Weiqiang;Song, Qingxin;Wang, Jia-Wei;Xu, Lin;Wu, Liang;Wu, Zhe;Xia, Rui;Xiao, Jun;Zhao, Yusheng;Zhai, Jixian;Zhong, Xuehua;Zhou, Dao-Xiu;Zhou, Ming;Zhou, Yue;Zhu, Bo;Liu, Qikun

    关键词：epigenetics; plant development; plant immunity; crop improvement; stress responses

  • The GABA-modulated energy metabolism reconfiguration positively regulates cotton (Gossypium hirsutum L.) responses during post-waterlogging recovery

    作者：Wang, Li;Zheng, Saiya;Li, Leyue;Liu, Xuanxuan;Feng, Liuchun;Liu, Xinyan;Huang, Qingqing;Zhang, Yibin;Yan, Gentu;Wang, Ning;Ma, Chunmei;Yan, Gentu;Wang, Ning

    关键词：Cotton; RNA-sequencing; Widely targeted metabolomics; Post-waterlogging stress; Alternative energy metabolism; Reactive oxygen species

  • Proteolysis and lipolysis induced by acidification of sesame seeds

    作者：Wang, Xinyu;Zhang, Caimeng;Kong, Xiangzhen;Hua, Yufei;Chen, Yeming;Wang, Lili;Wang, Lijuan

    关键词：Seeds; Acidification; Proteases; Protein storage vacuoles; Lipases; Oil bodies

  • Overexpression of the GmPM35 Gene Significantly Enhances Drought Tolerance in Transgenic Arabidopsis and Soybean

    作者：Wang, Xinyu;Li, Xinyang;Wang, Tianyu;Guo, Zhaohao;Li, Yan;Qiu, Wenxi;Guan, Shuyan;Zhang, Qi;Wang, Piwu;Li, Mingze;Liu, Siyan;Sun, Yao;Wang, Rui;Li, Yongyi;Guan, Shuyan;Zhang, Qi;Wang, Piwu;Liu, Siyan;Fan, Xuhong

    关键词：soybeans; Arabidopsis thaliana; LEA; GmPM35; drought tolerance; ROS

  • Honey Robbing: Causes, Impacts and Preventive Measures

    作者：Wang, Xinyu;Huang, Ting;Ji, Quanzhi;Zhao, Yazhou;Guo, Jun

    关键词：honey bee; honey robbing; behavioral characteristics; causes; hazards; occurrence mechanism

  • Spatiotemporal Transcriptomic Profiling Reveals the Dynamic Immunological Landscape of Alveolar Echinococcosis

    作者：Ou, Zhihua;Ren, Peidi;He, Fan;Chen, Jialing;Tan, Qiming;Chen, Qi;Zhang, Qian;Ou, Zhihua;Ren, Peidi;He, Fan;Chen, Jialing;Cai, Huimin;Li, Jiandong;Tan, Qiming;Chen, Qi;He, Xiuju;Zhao, Yanping;Zhang, Qian;Liang, Yingan;Li, Junhua;Li, Li;Zhou, Ting-Ting;Wu, Yao-Dong;Li, Xiu-Rong;Li, Wenhui;Zhang, Nian-Zhang;Chen, Wei-Gang;Sun, Jiwen;Wu, Yan-Tao;You, Jie;Tian, Xue-Qi;Fu, Bao-Quan;Cai, Xue-Peng;Jia, Wan-Zhong;Yan, Hong-Bin;Li, Li;Zhou, Ting-Ting;Wu, Yao-Dong;Li, Xiu-Rong;Li, Wenhui;Zhang, Nian-Zhang;Chen, Wei-Gang;Sun, Jiwen;Wu, Yan-Tao;You, Jie;Tian, Xue-Qi;Fu, Bao-Quan;Cai, Xue-Peng;Jia, Wan-Zhong;Yan, Hong-Bin;He, Fan;Chen, Jialing;Chen, Qi;Zhang, Qian;Cai, Huimin;Li, Jiandong;He, Xiuju;Zhao, Yanping;Liao, Sha;Yang, Huanming;Wang, Jian;Jin, Xin;Xu, Xun;Han, Xiumin;Tan, Qiming;Liang, Yingan;Hu, Guohai;Wang, Jian;Chen, Ao;Chen, Ao;Jin, Xin;Jin, Xin;Xu, Xun;Li, Junhua

    关键词：Echinococcus multilocularis; macrophages; neutrophils; single-cell RNA sequencing; spatial transcriptomics

  • Rootstock-mediated effects on vine performance and quality composition of 'Miguang' under protected cultivation in northern China

    作者：Wang, Xinyu;Liu, Changjiang;Jia, Nan;Yin, Yonggang;Han, Bin;Sun, Yan;Han, Shuli;Guo, Yong;Li, Minmin

    关键词：Rootstock; 'Miguang'; Growth; Yield; Aroma profiles; Grape

微信小程序