Identification of differentially expressed genes of Haloxylon ammodendron in response to salinity stress

文献类型： 外文期刊

第一作者： He, J. F.

作者： He, J. F.;Fang, Y. Y.;Wang, L. W.;Zhao, X. Q.;Fu, X. F.;Liu, H. K.;Zhao, J.;Lu, Z. X.

作者机构：

关键词： carbon metabolism; drought and salinity tolerance; heat shock proteins; hormone-related genes; NaCl; RNA-Seq; transcription factors

期刊名称：BIOLOGIA PLANTARUM （ 影响因子：1.747； 五年影响因子：2.146 ）

ISSN： 0006-3134

年卷期： 2019 年 63 卷

页码：

收录情况： SCI

摘要： Haloxylon ammodendron (C.A. Mey.), an endangered desert tree with excellent drought and salinity tolerance, provides a unique genotype to characterize and understand the tolerance mechanisms. In this study, four RNA-Seq libraries were constructed and sequenced from H. ammodendron under salinity stress. Total 12 027 differentially expressed genes (DEGs) were identified, in which 4 023, 3 517, 4 487 genes were differentially expressed under light salinity stress (200 mM NaCl), moderate salinity stress (400 mM NaCl), and severe salinity stress (800 mM NaCl), respectively. The up-regulated DEGs included several transcription factors (e.g., MYB and bHLH), hormone-related genes (e.g., cytochrome P450), protein kinases (e.g., Atpk2-Atpk19 like), and genes involved in carbon metabolism (e.g., UDP glycosyltransferase), osmotic regulation (e.g., proline transporter), and ubiquitin proteasome system (e.g., ubiquitin-conjugating enzymes). Heat shock proteins were identified as positive regulators of salinity tolerance in H. ammodendron. The expression patterns of 13 DEGs verified by real-time quantitative PCR were identically consistent with the variations in transcript abundance identified by RNA-Seq. Our results provide new insights into molecular mechanism of H. ammodendron in response to salinity stress.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序