Quantification of Enterocytozoon hepatopenaei (EHP) in Penaeid Shrimps from Southeast Asia and Latin America Using TaqMan Probe-Based Quantitative PCR

文献类型： 外文期刊

第一作者： Piamsomboon, Patharapol

作者： Piamsomboon, Patharapol;Choi, Seong-Kyoon;Park, Song;Choi, Seong-Kyoon;Hanggono, Bambang;Nuraini, Yani Lestari;Wati, Fatma;Tang, Kathy F. J.;Kwak, Dongmi;Rhee, Man Hee;Han, Jee Eun;Kim, Ji Hyung

作者机构：

关键词： Enterocytozoon hepatopenaei; qPCR; microsporidian; shrimp parasite

期刊名称：PATHOGENS （ 影响因子：3.492； 五年影响因子：4.066 ）

ISSN：

年卷期： 2019 年 8 卷 4 期

页码：

收录情况： SCI

摘要： We developed a qPCR assay based on the beta-tubulin gene sequence for the shrimp microsporidian parasite Enterocytozoon hepatopenaei (EHP). This assay reacted with the hepatopancreas (HP) of EHP-infected shrimps, and the highest copy numbers were found in HP and feces samples from Southeast Asian countries (10(6)-10(8) copies mg(-1)), while HP samples from Latin America, Artemia, and EHP-contaminated water showed lower amounts (10(1)-10(3) copies mg(-1) or mL(-1) of water). No false positive was found with the normal shrimp genome, live feeds, or other parasitic diseases. This tool will facilitate the management of EHP infection in shrimp farms.

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