Antler stem cell-conditioned medium stimulates regenerative wound healing in rats

文献类型： 外文期刊

第一作者： Rong, Xiaoli

作者： Rong, Xiaoli;Li, Chunyi;Rong, Xiaoli;Wang, Yimin;Rong, Xiaoli;Qi, Xiaoyan;Zhang, Guokun;Li, Chunyi;Chu, Wenhui;Zhang, Haiying;Wang, Yusu

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关键词： Antler stem cells; Conditioned medium; Cell proliferation; Scarless healing; Skin regeneration

期刊名称：STEM CELL RESEARCH & THERAPY （ 影响因子：6.832； 五年影响因子：7.153 ）

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年卷期： 2019 年 10 卷 1 期

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收录情况： SCI

摘要： Background: When the deer antler is cast, it leaves a cutaneous wound that can achieve scarless healing due to the presence of antler stem cells (ASCs). This provides an opportunity to study regenerative wound healing. Methods: In this study, we investigated the therapeutic effects and mechanism of antler stem cell-conditioned medium (ASC-CM) on cutaneous wound healing in rats. In vitro, we investigated the effects of the ASC-CM on proliferation of HUVEC and NIH-3T3 cell lines. In vivo, we evaluated the effects of ASC-CM on cutaneous wound healing using full-thickness skin punch-cut wounds in rats. Results: The results showed that ASC-CM significantly stimulated proliferation of the HUVEC and NIH-3T3 cells in vitro. In vivo, completion of healing of the rat wounds treated with ASC-CM was on day 16 (+/- 3 days), 9 days (+/- 2 days) earlier than the control group (DMEM); the area of the wounds treated with ASC-CM was significantly smaller (p < 0.05) than the two control groups. Further molecular characterization showed that the ratios of Col3A1/Col1A2, TGF-beta 3/TGF-beta 1, MMP1/TIMP1, and MMP3/TIMP1 significantly increased (p < 0.01) in the healed tissue in the ASC-CM group. Conclusions: In conclusion, ASC-CM effectively accelerated the wound closure rate and enhanced the quality of healing, which might be through transforming wound dermal fibroblasts into the fetal counterparts. Therefore, the ASC-CM may have potential to be developed as a novel cell-free therapeutic for scarless wound healing.

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