Interferon regulatory factor 2 plays a positive role in interferon gamma expression in golden pompano, Trachinotus ovatus (Linnaeus 1758)

文献类型： 外文期刊

第一作者： Zhu, Ke-Cheng

作者： Zhu, Ke-Cheng;liu, Bao-Suo;Zhang, Nan;Guo, Hua-Yang;Gue, Liang;Jiang, Shi-Gui;Zhang, Dian-Chang;Zhu, Ke-Cheng;liu, Bao-Suo;Zhang, Nan;Guo, Hua-Yang;Gue, Liang;Jiang, Shi-Gui;Zhang, Dian-Chang;Zhu, Ke-Cheng;liu, Bao-Suo;Zhang, Nan;Guo, Hua-Yang;Gue, Liang;Jiang, Shi-Gui;Zhang, Dian-Chang

作者机构：

关键词： Trachinotus ovatus; Promoter activity; EMSA; IRF2; Type II IFN

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN： 1050-4648

年卷期： 2020 年 96 卷

页码：

收录情况： SCI

摘要： In fish, interferon (IFN) regulatory factor 2 (IRF2) is a regulator of the type I IFN-dependent immune response, thereby playing a crucial role in innate immunity. However, the specific mechanism by which IRF2 regulates type II IFN in fish remains unclear. In the present study, first, to analyse the potential role of golden pompano (Trachinotus ovatus) IRF2 (ToIRF2) in the immune response, the mRNA level of ToIRF2 was detected by quantitative real-time polymerase chain reaction (qRT-PCR) after parasite infection. ToIRF2 was upregulated at early time points in both local infection sites (skin and gill) and system immune tissues (liver, spleen, and head-kidney) after stimulation with Cryptocaryon irritans. Second, to investigate the modulation effect of ToIRF2 on type II IFN (interferon gamma, IFN gamma) expression, a promoter analysis was performed using progressive deletion mutations of TolFiV gamma. The expression level of IFN gamma-5 was highest among the five truncated mutants in response to ToIRF2, indicating that the core promoter region was located from - 189 bp to +120 bp, which included the IRF2 binding sites. Mutation analyses showed that the activity of the TolFN gamma promoter dramatically decreased after the targeted mutation of the M1, M2 or M3 binding sites. Additionally, electrophoretic mobile shift assay (EMSA) confirmed that IRF2 interacted with the M1 binding site in the TolFN gamma promoter region to dominate TolEN gamma expression. Finally, overexpressing ToIRF2 in vitro notably increased ToIFN gamma and the transcription of several type II IFN/IRF-based signalling pathway genes. These results suggested that ToIRF2 might be involved in the host defence against C. irritans infection and contribute to a better understanding of the transcriptional mechanisms by which ToIRF2 regulates type II IFN in fish.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序