Induction of gyno-tetraploidy in Japanese flounder Paralichthys olivaceus

文献类型： 外文期刊

第一作者： Wang, Guixing

作者： Wang, Guixing;Wang, Guixing;Zhang, Xiaoyan;Sun, Zhaohui;Zhao, Yaxian;Du, Wei;Hou, Jilun;Wang, Yufen;Cui, Jianbin

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关键词： gynogenesis; polyploidy; chromosome manipulation; mosaic

期刊名称：JOURNAL OF OCEANOLOGY AND LIMNOLOGY （ 影响因子：1.265； 五年影响因子：1.276 ）

ISSN： 2096-5508

年卷期： 2020 年 38 卷 1 期

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收录情况： SCI

摘要： Tetraploid fish are important for mass production of triploids in polyploid breeding. In this study, we reported a novel protocol for artificial induction of tetraploidy by a combination of cold shock and hydrostatic pressure administered to gynogenetically developed eggs in Japanese flounder Paralichthys olivaceus. The induction was carried out by activating the eggs with UV-irradiated sperm of red sea bream Pagrus major, administering a cold shock (0 degrees C, 5 to 45 min) 3 min after fertilization to inhibit second polar body exclusion, incubating the eggs for 60 min at 17 degrees C, and treating them with a 650 kg/cm(2) hydrostatics pressure shock for 6 min. We named the embryos gyno-tetraploids that developed from eggs after such treatments. The hatching rate of the gyno-tetraploids ranged from 20.99%+/- 3.66% to 36.01%+/- 2.79%, and the tetraploid rate ranged from 80.00% to 100.00%. All-maternal inheritance was verified using 6 high-recombination-rate microsatellite markers. This method successfully induced gyno-tetraploidy. The successful induction of gyno-tetraploidy lays the foundation for triploidization of new varieties with improved economic traits of interest that can benefit commercial culture.

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