Purification and Characterization of a Novel Lipase from Antarctic Krill

文献类型： 外文期刊

第一作者： Chen Xin

作者： Chen Xin;Jiang Yihui;Chen Xin;Wang Chunlan;Xu Jiakun;Wang Fang;Jiang Yihui;Chen Yixuan;Zhao Xianyong;Xu Jiakun;Zhao Xianyong;Chen Yixuan

作者机构：

关键词： Antarctic krill; lipase; isolation and purification; enzymology properties

期刊名称：JOURNAL OF OCEAN UNIVERSITY OF CHINA （ 影响因子：0.913； 五年影响因子：1.012 ）

ISSN： 1672-5182

年卷期： 2020 年 19 卷 1 期

页码：

收录情况： SCI

摘要： Lipase from Antarctic krill, with a molecular weight of 71.27kDa, was purified with ammonium sulfate precipitation and a series of chromatographic separations over ion exchange (DEAE) and gel filtration columns (Sephacryl S-100), resulting in 5.2% recovery with a 22.4-fold purification ratio. The optimal pH and temperature for enzyme activity were 8.0 and 45 degrees C, respectively. Purified lipase had K-m and V-max values of 3.27 mmol L-1 and 2.4 Umg(-1), respectively, using p-nitrophenyl laurate as the substrate. Li-pase activity was enhanced by adding Ca2+ and Mg2+ ions in the concentration ranges of 0-0.5 mmol L-1 and 0-0.3mmol L-1, respectively, while the activity was inhibited by a further increase in these ion concentrations. Fe3+ and Cu2+ ions showed obvious inhibitory effects on enzyme activity, and the inhibition rates were 71.8% and 53.3% when the ion concentrations were 0.5 mmol L-1.

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