A thermostable serralysin inhibitor from marine bacterium Flavobacterium sp YS-80-122

文献类型： 外文期刊

第一作者： Liang Pengjuan

作者： Liang Pengjuan;Li Shangyong;Wang Kun;Wang Fang;Xing Mengxin;Hao Jianhua;Sun Mi;Liang Pengjuan;Li Shangyong;Xing Mengxin;Hao Jianhua;Sun Mi;Wang Kun

作者机构：

关键词： serralysin inhibitor; sequence analysis; kinetic parameter; thermostable

期刊名称：JOURNAL OF OCEANOLOGY AND LIMNOLOGY （ 影响因子：1.265； 五年影响因子：1.276 ）

ISSN： 2096-5508

年卷期： 2018 年 36 卷 2 期

页码：

收录情况： SCI

摘要： Serralysin inhibitors have been proposed as potent drugs against many diseases and may help to prevent further development of antibiotic-resistant pathogenic bacteria. In this study, a novel serralysin inhibitor gene, lupI, was cloned from the marine bacterium Flavobacterium sp. YS-80-122 and expressed in Escherichia coli. The deduced serralysin inhibitor, LupI, shows < 40% amino acid identity to other reported serralysin inhibitors. Multiple sequence alignment and phylogenetic analysis of LupI with other serralysin inhibitors indicated that LupI was a novel type of serralysin inhibitor. The inhibitory constant for LupI towards its target metalloprotease was 0.64 mu mol/L. LupI was thermostable at high temperature, in which 35.6%-90.7% of its inhibitory activity was recovered after treatment at 100 degrees C for 1-60 min followed by incubation at 0 degrees C. This novel inhibitor may represent a candidate drug for the treatment of serralysin-related infections.

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