Small Molecular Contaminant and Microorganism Can Be Simultaneously Detected Based on Nanobody-Phage: Using Carcinogen Aflatoxin and Its Main Fungal Aspergillus Section Flavi spp. in Stored Maize for Demonstration

文献类型： 外文期刊

第一作者： Ren, Xianfeng

作者： Ren, Xianfeng;Yue, Xiaofeng;Mwakinyali, Silivano Edson;Zhang, Wen;Zhang, Qi;Li, Peiwu;Ren, Xianfeng;Yue, Xiaofeng;Mwakinyali, Silivano Edson;Zhang, Wen;Zhang, Qi;Li, Peiwu;Yue, Xiaofeng;Mwakinyali, Silivano Edson;Zhang, Wen;Zhang, Qi;Li, Peiwu;Li, Peiwu;Li, Peiwu

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关键词： real-time PCR; aflatoxin; Aspergillus; nanobody-phage; Nor-1 gene

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）

ISSN： 1664-302X

年卷期： 2020 年 10 卷

页码：

收录情况： SCI

摘要： Simultaneous detection technology has become a hot topic in analytical chemistry; however, very few reports on how to simultaneously detect small molecular contaminants and microorganisms have been in place. Aflatoxins are a group of highly toxic and carcinogenic compounds, which are produced mainly by Aspergillus flavus and Aspergillus parasiticus from section Flavi responsible for aflatoxin accumulation in stored cereals. Both aflatoxins and Aspergillus section Flavi were used to demonstrate the duplex real-time RCR method of simultaneously detecting small molecular contaminants and microorganisms. The detection of aflatoxins and Aspergillus section Flavi was carried out depending on the anti-idiotypic nanobody-phage V2-5 and aflatoxin-synthesis related gene nor-1 (=aflD), respectively. The quantitative standard curves for simultaneous detection of aflatoxins and Aspergillus section Flavi were constructed, with detection limits of 0.02 ng/ml and 8 x 10(2) spores/g, respectively. Naturally contaminated maize samples (n = 25) were analyzed for a further validation. The results were in good agreement between the new developed method and the referential methods (high-performance liquid chromatography and the conventional plating counts).

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