Feeding Delivery of dsHvSnf7 Is a Promising Method for Management of the Pest Henosepilachna vigintioctopunctata (Coleoptera: Coccinellidae)

文献类型： 外文期刊

第一作者： Lu, Jing

作者： Lu, Jing;Liu, Zhuoqi;Guo, Wei;Guo, Mujuan;Chen, Shimin;Pan, Huipeng;Lu, Jing;Liu, Zhuoqi;Guo, Wei;Guo, Mujuan;Chen, Shimin;Pan, Huipeng;Lu, Jing;Liu, Zhuoqi;Guo, Wei;Guo, Mujuan;Chen, Shimin;Pan, Huipeng;Li, Huali;Yang, Chunxiao;Zhang, Youjun

作者机构：

关键词： Henosepilachna vigintioctopunctata; HvSnf7; feeding RNAi; mortality; ultrastructural change

期刊名称：INSECTS （ 影响因子：2.769； 五年影响因子：3.046 ）

ISSN：

年卷期： 2020 年 11 卷 1 期

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收录情况： SCI

摘要： RNA interference (RNAi) techniques have emerged as powerful tools in the development of novel management strategies for the control of insect pests, such as Henosepilachna vigintioctopunctata, which is a major solanaceous pest in Asia. Our results showed that levels of HvSnf7 expression were greater in larval midguts than in other tissues. Silencing of HvSnf7 led to greater H. vigintioctopunctata mortality rates and appeared to be time- and partially dose-dependent. Bacterially expressed dsHvSnf7 that was applied to detached plant leaves caused 98, 88, and 60% mortality in 1st and 3rd instars, and adults after 10, 12, and 14 d, respectively; when applied to living plants, bacterially expressed dsHvSnf7 led to mortality in 1st and 3rd instars, with no effect on adults. Bacterially expressed dsHvSnf7 led to improved plant protection against H. vigintioctopunctata. Ultrastructural changes caused by HvSnf7-RNAi in larval midguts showed extensive loss of cellular contents that indicate loss of membrane integrity. This study indicate that HvSnf7 potentially can be used as RNAi target gene for controlling of H. vigintioctopunctata.

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