Dynamic accumulation of fatty acids in duck (Anas platyrhynchos) breast muscle and its correlations with gene expression

文献类型： 外文期刊

第一作者： Fan, Wenlei

作者： Fan, Wenlei;Liu, Hehe;Meng, Qingshi;Xu, Yaxi;Zhou, Zhengkui;Hou, Shuisheng;Fan, Wenlei;Liu, Hehe;Meng, Qingshi;Xu, Yaxi;Zhou, Zhengkui;Hou, Shuisheng;Fan, Wenlei;Liu, Wenjing;Wang, Baowei;Fan, Wenlei;Guo, Yuming

作者机构：

关键词： Lipid metabolism; Fatty acid profile; Duck; Breast muscle; Transcriptome

期刊名称：BMC GENOMICS （ 影响因子：3.969； 五年影响因子：4.478 ）

ISSN： 1471-2164

年卷期： 2020 年 21 卷 1 期

页码：

收录情况： SCI

摘要： Background Fatty acid composition contributes greatly to the quality and nutritional value of meat. However, the molecular regulatory mechanisms underlying fatty acid accumulation in poultry have not yet been cleared. The aims of this study were to characterize the dynamics of fatty acid accumulation in duck breast muscle and investigate its correlations with gene expression. Results Here, we analyzed the fatty acid profile and transcriptome of breast muscle derived from Pekin ducks and mallards at the ages of 2 weeks, 4 weeks, 6 weeks and 8 weeks. Twenty fatty acids were detected in duck breast muscle, with palmitic acid (C16:0, 16.6%similar to 21.1%), stearic acid (C18:0, 9.8%similar to 17.7%), oleic acid (C18:1n-9, 15.7%similar to 33.8%), linoleic acid (C18:2n-6, 10.8%similar to 18.9%) and arachidonic acid (C20:4n-6, 11.7%similar to 28.9%) as the major fatty acids. Our results showed that fatty acid composition was similar between the two breeds before 6 weeks, but the compositions diverged greatly after this point, mainly due to the stronger capacity for C16:0 and C18:1n-9 deposition in Pekin ducks. By comparing the multistage transcriptomes of Pekin ducks and mallards, we identified 2025 differentially expressed genes (DEGs). Cluster analysis of these DEGs revealed that the genes involved in oxidative phosphorylation, fatty acid degradation and the PPAR signaling pathway were upregulated in mallard at 8 weeks. Moreover, correlation analysis of the DEGs and fatty acid composition traits suggested that the DEGs involved in lipogenesis, lipolysis and fatty acid beta-oxidation may interact to influence the deposition of fatty acids in duck breast muscle. Conclusions We reported the temporal progression of fatty acid accumulation and the dynamics of the transcriptome in breast muscle of Pekin ducks and mallards. Our results provide insights into the transcriptome regulation of fatty acid accumulation in duck breast muscle, and will facilitate improvements of fatty acid composition in duck breeding.

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