Anti-apoptotic Effect of Taurine on Schwann Cells Exposed to High Glucose In Vitro

文献类型： 外文期刊

第一作者： Li, Kaixin

作者： Li, Kaixin;Inam-u-Llah;Shi, Xiaoxia;Zhang, Mengren;Wu, Pingan;Li, Shuangyue;Piao, Fengyuan;Suleman, Raheel;Nisar, Azhar

作者机构：

关键词： High glucose; Apoptosis; Schwann cells; Schwann cells; Anti-apoptotic effect

期刊名称：TAURINE 11 （ 影响因子：2.622； 五年影响因子：3.049 ）

ISSN： 0065-2598

年卷期： 2019 年 1155 卷

页码：

收录情况： SCI

摘要： It was reported that apoptosis of Schwann cells could increase in the diabetic rats. The studies showed that taurine inhibited apoptosis in a variety of cells. However, there were few reports on studying the protection of taurine against apoptosis of Schwann cells induced by high glucose (HG) and the underlying mechanism. In our study, the cells were divided into five groups: Control: the normal medium; HG group: 50 mM high glucose; T1: 50 mM high glucose+Taurine (10 mM) group; T2: 50 mM high glucose+Taurine (20 mM) group; T3: 50 mM high glucose+Taurine (40 mM) group. We used MTT and Tunel assays to measure the cell viability and apoptosis, respectively. Then, we also used western blotting to detect the protein levels of apoptosis-related protein. The results demonstrate that taurine promoted cell viability and decreased apoptosis in RSC96 cells exposed to HG. Furthermore, taurine markedly improved imbalance of Bax and Bcl-2, inhibited the translocation of Cytochrome C (Cyt C) from mitochondria to cytosol and reduced caspase-3 activity in HG-induced RSC96 cells. Our results indicate that taurine protect against apoptosis of Schwann cells induced by HG via inhibiting mitochondria-dependent caspase-3 pathway.

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