Purification, Identification and Functional Analysis of a Novel Immunomodulatory Peptide from Silkworm Pupa Protein

文献类型： 外文期刊

第一作者： Li, Zhiyong

作者： Li, Zhiyong;Zhao, Shan;Xin, Xiangdong;Zhang, Bei;Thomas, Attaribo;Charles, Asakiya;Gui, Zhongzheng;Lee, Kwang Sik;Jin, Byung Rae;Gui, Zhongzheng

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关键词： Silkworm pupa protein; Immunomodulatory peptide; Purification; Identification

期刊名称：INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS （ 影响因子：1.931； 五年影响因子：1.712 ）

ISSN： 1573-3149

年卷期： 2020 年 26 卷 1 期

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收录情况： SCI

摘要： In this study, we isolated and characterized an immunomodulatory peptide from silkworm (Bombyx mori) pupa protein hydrolysates. Ultrasound-pretreated hydrolysates were prepared by alcalase digestion and their pro-proliferative activity was assessed with the splenic lymphocyte proliferation assay. Peptide fractions exhibiting the highest activity were purified by Sephadex-G100 and -G15 gel filtration chromatography. The structure of the purified peptides was analyzed using Liquid Chromatography Electrospray Ionisation Tandem Mass Spectrometry (LC-ESI-MS/MS); their digestive stability and their effects of the expression on immune-related cytokines were also evaluated. We purified a novel immunomodulatory peptide with a molecular weight of about 441.06 Da. The amino acid sequence was Asp-His-Ala-Val (DHAV). The splenocyte proliferation rate was 91.1% (P < 0.05) in the presence of 100 mu g/mL purified peptide. DHAV was stable in the presence of the gastrointestinal proteases pepsin and trypsin, and stimulated the expression of immune-related factors including interleukin-6 and -12, nuclear factor-kappa B, cyclin D1, and cyclin-dependent kinase 4. Thus, DHAV purified from silkworm pupa protein hydrolysate has immunomodulatory activity and potential therapeutic value.

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