Molecular Mapping and Candidate Gene Analysis for GA(3) Responsive Short Internode in Watermelon (Citrullus lanatus)

文献类型： 外文期刊

第一作者： Gebremeskel, Haileslassie

作者： Gebremeskel, Haileslassie;Dou, Junling;Li, Bingbing;Zhao, Shengjie;Muhammad, Umer;Lu, Xuqiang;He, Nan;Liu, Wenge

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关键词： candidate gene; BSA-Seq; GA 3 beta-hydroxylase; fine mapping; cytological analysis

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

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年卷期： 2020 年 21 卷 1 期

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收录情况： SCI

摘要： Plants with shorter internodes are suitable for high-density planting, lodging resistance and the preservation of land resources by improving yield per unit area. In this study, we identified a locus controlling the short internode trait in watermelon using Zhengzhouzigua (long internode) and Duan125 (short internode) as mapping parents. Genetic analysis indicated that F-1 plants were consistent with long internode plants, which indicates that the long internode was dominant over the short internode. The observed F-2 and BC1 individuals fitted the expected phenotypic segregation ratios of 3:1 and 1:1, respectively. The locus was mapped on chromosome 9 using a bulked segregant analysis approach. The region was narrowed down to 8.525 kb having only one putative gene, Cla015407, flanking by CAPS90 and CAPS91 markers, which encodes gibberellin 3 beta-hydroxylase (GA 3 beta-hydroxylase). The sequence alignment of the candidate gene between both parents revealed a 13 bp deletion in the short internode parent, which resulted in a truncated protein. Before GA(3) application, significantly lower GA(3) content and shorter cell length were obtained in the short internode plants. However, the highest GA(3) content and significant increase in cell length were observed in the short internode plants after exogenous GA(3) application. In the short internode plants, the expression level of the Cla015407 was threefold lower than the long internode plants in the stem tissue. In general, our results suggested that Cla015407 might be the candidate gene responsible for the short internode phenotype in watermelon and the phenotype is responsive to exogenous GA(3) application.

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