A loss-of-function mutant allele of a glycosyl hydrolase gene has been co-opted for seed weight control during soybean domestication

文献类型： 外文期刊

第一作者： Wei, Siming

作者： Wei, Siming;Yong, Bin;An, Zhenghong;Wang, Yan;Li, Bingbing;Yang, Ce;Zhu, Weiwei;He, Chaoying;Wei, Siming;Yong, Bin;An, Zhenghong;Wang, Yan;Li, Bingbing;Yang, Ce;Zhu, Weiwei;He, Chaoying;Wei, Siming;Yong, Bin;An, Zhenghong;Li, Bingbing;Yang, Ce;Zhu, Weiwei;He, Chaoying;Jiang, Hongwei;Chen, Qingshan;Jiang, Hongwei;He, Chaoying

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关键词： domestication; frameshift mutation; QTL; seed weight; soybean; to gain by loss

期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 影响因子：11.4； 五年影响因子：10.1 ）

ISSN： 1672-9072

年卷期： 2023 年 65 卷 11 期

页码：

收录情况： SCI

摘要： The resultant DNA from loss-of-function mutation can be recruited in biological evolution and development. Here, we present such a rare and potential case of "to gain by loss" as a neomorphic mutation during soybean domestication for increasing seed weight. Using a population derived from a chromosome segment substitution line of Glycine max (SN14) and Glycine soja (ZYD06), a quantitative trait locus (QTL) of 100-seed weight (qHSW) was mapped on chromosome 11, corresponding to a truncated & beta;-1, 3-glucosidase (& beta;Glu) gene. The novel gene hsw results from a 14-bp deletion, causing a frameshift mutation and a premature stop codon in the & beta;Glu. In contrast to HSW, the hsw completely lost & beta;Glu activity and function but acquired a novel function to promote cell expansion, thus increasing seed weight. Overexpressing hsw instead of HSW produced large soybean seeds, and surprisingly, truncating hsw via gene editing further increased the seed size. We further found that the core 21-aa peptide of hsw and its variants acted as a promoter of seed size. Transcriptomic variation in these transgenic soybean lines substantiated the integration hsw into cell and seed size control. Moreover, the hsw allele underwent selection and expansion during soybean domestication and improvement. Our work cloned a likely domesticated QTL controlling soybean seed weight, revealed a novel genetic variation and mechanism in soybean domestication, and provided new insight into crop domestication and breeding, and plant evolution.

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