Transmission Characteristics of Wheat Yellow Striate Virus by its Leafhopper Vector Psammotettix alienus

文献类型： 外文期刊

第一作者： Du, Zhenzhen

作者： Du, Zhenzhen;Fu, Yumei;Liu, Yan;Wang, Xifeng

作者机构：

关键词： Wheat yellow striate virus (WYSV); Nucleorhabdovirus; Psammotettix alienus; transmission; propagative

期刊名称：PLANT DISEASE （ 影响因子：4.438； 五年影响因子：4.7 ）

ISSN： 0191-2917

年卷期： 2020 年 104 卷 1 期

页码：

收录情况： SCI

摘要： Wheat yellow striate virus (WYSV), which is found in wheat fields of Northwest China and transmitted by leafhopper vector Psammotettix alienus, is a tentative new species in the genus Nucleorhabdovirus. Although the insect vector and host range of WYSV have been characterized, many aspects of the acquisition and transmission processes by its insect vector have not been elucidated. Here, the transmission parameters of WYSV by P. alienus were determined using wheat cv. Yangmai 12 as the indicator plant under a controlled temperature (23 +/- 1 degrees C) and photoperiod (16 h of light). The results showed that the minimum periods for acquisition were 5 min and 10 min for inoculation access. The latent period for successful transmission was most commonly 16 to 20 days (minimum, 10 days; maximum, 22 days). The quantitative reverse-transcriptase PCR results indicated that the WYSV titer increased with time after acquisition, suggesting that WYSV can replicate in P. alienus. Notably, female P. alienus transovarially transmitted the virus to next generations at relatively high efficiency. Electron microscopy of the WYSV-infected leafhopper revealed bacilliform particles aggregated in the cytoplasm of the salivary gland and midgut tissues. Our present studies suggested that acquisition and transmission of WYSV by P. alienus is consistent with a propagative, circulative, and persistent mode of transmission. Details regarding transmission competencies and distribution of WYSV in P. alienus will provide a basis for designing preventive measures.

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[1]Transmission Characteristics of Wheat Yellow Striate Virus by its Leafhopper Vector Psammotettix alienus. Du, Zhenzhen,Fu, Yumei,Liu, Yan,Wang, Xifeng. 2020

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