miR-144 Mediates High Fat Induced Changes of Cholesterol Metabolism via Direct Regulation of C/EBP alpha in the Liver and Isolated Hepatocytes of Yellow Catfish

文献类型： 外文期刊

第一作者： Chen, Guanghui

作者： Chen, Guanghui;Wu, Kun;Zhao, Tao;Ling, Shicheng;Luo, Zhi;Liu, Wei;Luo, Zhi

作者机构：

关键词： dietary lipid; high-throughput sequencing; microRNAs; cholesterol metabolism; transcriptional regulation

期刊名称：JOURNAL OF NUTRITION （ 影响因子：4.798； 五年影响因子：5.718 ）

ISSN： 0022-3166

年卷期： 2020 年 150 卷 3 期

页码：

收录情况： SCI

摘要： Background: microRNAs (miRNAs) post-transcriptionally regulate gene expression and act as important modulators of cholesterol homeostasis. Objective: The study explores the mechanism by which miRNAs mediate high fat-induced changes of cholesterol metabolism in yellow catfish. Methods: Yellow catfish (weight: 3.79 +/- 0.16 g, 3 mo old, mixed sex) were fed 2 diets containing lipids at 11.3% (control (CON)) or 15.4% [high-fat diet (HFD)] (by weight) for 8 wk. Cholesterol content was measured; hematoxylin-eosin (H&E) staining, qPCR assays, and small RNA sequencing were conducted in the liver. Hepatocytes were isolated from separate, untreated fish and incubated for 24 h in control solution or palmitic acid (PA; 0.25 mM)/oleic acid (OA; 0.5 mM) after 4 h pretreatment with or without miR-144 inhibitor/mimic (40 nM). Cholesterol content was measured; qPCR assays and Western blotting were conducted in the hepatocytes. HEK293T cells were co-transfected with plasmids to validate miR144 target genes. The promoter activities of miR-144 were analyzed in HEK293T cells with PA (0.25 mM) or OA (0.25 or 0.5 mM) treatment for 24 h. Luciferase activity assays, electrophoretic mobility shift assay, and Western blotting were conducted in HEK293T cells. Results: Compared with CON, HFD induced hepatic cholesterol accumulation (31.5%), and upregulated miR-144 expression (8.40-fold, P < 0.05). miR-144 directly targeted hydroxymethylglutaryl-CoA reductase (hmgcr), cholesterol 7 alpha-monooxygenase A1 (cyp7a1), and adenosine triphosphate binding cassette transporter A1 (abca1) in HEK293T cells. In the hepatocytes of yellow catfish, miR-144 inversely regulated the expression of hmgcr, cyp7a1, and abca1 (30.3-78.5%, P < 0.05); loss of miR-144 function alleviated PA- or OA-induced cholesterol accumulation (19.5-61.1%, P< 0.05). We also characterized the C/EBP alpha binding site in the miR-144 promoter, and found that C/EBP alpha positively regulated miR144 expression through binding to the miR-144 promoter. Conclusions: miR-144 mediated HFD-induced changes in the liver and hepatocytes of yellow catfish, suggesting a possible mechanism for HFD-induced dysfunction in cholesterol metabolism.

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