Heterologous expression of the ThIPK2 gene enhances drought resistance of common wheat

文献类型： 外文期刊

第一作者： Zhang Shu-juan

作者： Zhang Shu-juan;Li Yu-lian;Song Guo-qi;Gao Jie;Zhang Rong-zhi;Li Wei;Chen Ming-li;Li Gen-ying

作者机构：

关键词： codon-optimized; drought; rd29A; ThIPK2; Triticum aestivum L

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2020 年 19 卷 4 期

页码：

收录情况： SCI

摘要： ThIPK2 is an inositol polyphosphate kinase gene cloned from Thellungiella halophila that participates in diverse cellular processes. Drought is a major limiting factor in wheat (Triticum aestivum L.) production. The present study investigated whether the application of the ThIPK2 gene could increase the drought resistance of transgenic wheat. The codon-optimized ThIPK2 gene was transferred into common wheat through Agrobactetium-mediated transformation driven by either a constitutive maize ubiquitin promoter or a stress-inducible rd29A promoter from Arabidopsis. Molecular characterization confirmed the presence of the foreign gene in the transformed plants. The transgenic expression of ThIPK2 in wheat led to significantly improve drought tolerance compared to that observed in control plants. Compared to the wild type (WT) plants, the transgenic plants showed higher seed germination rates, better developed root systems, a higher relative water content (RWC) and total soluble sugar content, and less cell membrane damage under drought stress conditions. The expression profiles showed different expression patterns with the use of different promoters. The codon-optimized ThIPK2 gene is a candidate gene to enhance wheat drought stress tolerance by genetic engineering.

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