MERTK is a host factor that promotes classical swine fever virus entry and antagonizes innate immune response in PK-15 cells

文献类型： 外文期刊

第一作者： Zheng, Guanglai

作者： Zheng, Guanglai;Li, Lian-Feng;Zhang, Yuexiu;Qu, Liang;Wang, Wenjing;Li, Miao;Yu, Shaoxiong;Zhou, Mo;Luo, Yuzi;Sun, Yuan;Li, Su;Qiu, Hua-Ji;Munir, Muhammad

作者机构：

关键词： MERTK; TAM receptors; classical swine fever virus; entry; innate immune response

期刊名称：EMERGING MICROBES & INFECTIONS （ 影响因子：7.163； 五年影响因子：7.329 ）

ISSN：

年卷期： 2020 年 9 卷 1 期

页码：

收录情况： SCI

摘要： Classical swine fever virus (CSFV) is a member of the genus Pestivirus in the Flaviviridae family. To date, the host factors required for CSFV entry remain poorly characterized. To identify the functional membrane protein(s) involved in CSFV infection, we analyzed the transcriptomic data from previous studies describing gene expression profiles for CSFV, and found twelve novel candidate proteins. One of these proteins, MERTK, significantly reduced CSFV protein expression by RNA interference screening using a recombinant CSFV that contains a luciferase reporter to measure CSFV protein expression. Furthermore, our results demonstrated that either anti-MERTK antibodies or soluble MERTK ectodomain could reduce CSFV infection in PK-15 cells in a dose-dependent manner. Mechanistically, MERTK interacted with the E2 protein of CSFV and facilitated virus entry. After virus entry, MERTK downregulates of mRNA expression of IFN-beta and promotes CSFV infection. Interestingly, the soluble MERTK ectodomain could also reduce the infection of bovine viral diarrhea virus (BVDV), another pestivirus. Taken together, our results suggested that MERTK is a CSFV entry factor that synergistically dampens innate immune responses in PK-15 cells and is also involved in BVDV infection.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序