Optimization of extraction of total trans-resveratrol from peanut seeds and its determination by HPLC

文献类型： 外文期刊

第一作者： Xu, Siliang

作者： Xu, Siliang;Luo, Huaiyong;Chen, Haiwen;Guo, Jianbin;Yu, Bolun;Zhang, Huan;Li, Weitao;Chen, Weigang;Zhou, Xiaojin;Huang, Li;Liu, Nian;Lei, Yong;Liao, Boshou;Jiang, Huifang

作者机构：

关键词： high performance liquid chromatography; peanut seed; response surface methodology; trans-resveratrol

期刊名称：JOURNAL OF SEPARATION SCIENCE （ 影响因子：3.645； 五年影响因子：2.943 ）

ISSN： 1615-9306

年卷期： 2020 年 43 卷 6 期

页码：

收录情况： SCI

摘要： Resveratrol, a stilbene phytoalexin in plants, is believed to benefit human health. In this study, an optimized enzyme-assisted method was developed to extract the total content of trans-resveratrol (free or combined with glucose) in peanut seeds, followed by detection using high-performance liquid chromatography. The extraction process was optimized by Box-Behnken design and response surface methodology. The optimized enzyme concentration, digestion time, pH, and temperature were 3.02 g/L, 57.06 min, 5.88, and 51.05 degrees C, respectively. Validation tests indicated that the experimental yield of trans-resveratrol was 0.183 +/- 0.007 mu g/g with a relative standard deviation of 3.87% (n = 5) under the optimal condition, which was closely agreed with the predicted value (0.182 mu g/g). The recoveries obtained from the spiked samples were varied from 89.4 to 103.9%. Therefore, this study will provide a useful method for quantification of total trans-resveratrol in peanut seeds.

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[1]Optimization of extraction of total trans-resveratrol from peanut seeds and its determination by HPLC. Xu, Siliang,Luo, Huaiyong,Chen, Haiwen,Guo, Jianbin,Yu, Bolun,Zhang, Huan,Li, Weitao,Chen, Weigang,Zhou, Xiaojin,Huang, Li,Liu, Nian,Lei, Yong,Liao, Boshou,Jiang, Huifang.

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