Interaction between Translocation-associated membrane protein 1 and sigma C protein of novel duck reovirus controls virus infectivity

文献类型： 外文期刊

第一作者： Xiao, Rong

作者： Xiao, Rong;Sun, Jiahui;Ding, Mingyang;Li, Chuanfeng;Zhu, Jie;Liu, Guangqing;Chen, Zongyan;Xiao, Rong;Zhou, Hailong;Mi, Xiaoyun;Ma, Wenge

作者机构：

关键词： Novel duck reovirus; sigma c protein; TRAM1; Protein interaction; siRNA; Replication

期刊名称：VIRUS GENES （ 影响因子：2.332； 五年影响因子：2.0 ）

ISSN： 0920-8569

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页码：

收录情况： SCI

摘要： Novel duck reovirus (NDRV), the prototype strain of the species Avian orthoreovirus (ARV), is associated with high mortality in Pekin ducklings. sigma C is an outer capsid protein encoded by the S1 genome segment of NDRV which mediates attachment to host cells. Our previous studies using immunoprecipitation and mass spectrometry found that sigma C coprecipitated with some host proteins including Translocation-associated membrane protein 1 (TRAM1). However, the interaction between sigma C and TRAM1 has not been further confirmed experimentally. In this study, we utilized coimmunoprecipitation assays, glutathione S-transferase pull-down, and confocal microscopy to confirm the interaction between sigma C and TRAM1. In addition, knockdown of TRAM1 using siRNA and overexpression of TRAM1 gene were conducted to explore its effect on virus replication. The result showed that TRAM1 silencing benefits while overexpression inhibits viral replication. This study confirms the important role TRAM1 during NDRV infection which can help develop new approaches for NDRV disease prevention and control.

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