Expression profile of genes involved in ramie flavonoids biosynthesis pathway and regulation of flavanone 3-hydroxylase (BnF3H) in response to aquatic environment

文献类型： 外文期刊

第一作者： Gao, Gang

作者： Gao, Gang;Chen, Ping;Chen, Jikang;Chen, Kunmei;Xiong, Heping;Yu, Chunming;Zhu, Aiguo

作者机构：

关键词： Flavanone 3-hydroxylase; Flavonoid biosynthesis; Hydroponic system; Adventitious roots; Ramie

期刊名称：ACTA PHYSIOLOGIAE PLANTARUM （ 影响因子：2.354； 五年影响因子：2.711 ）

ISSN： 0137-5881

年卷期： 2020 年 42 卷 4 期

页码：

收录情况： SCI

摘要： Flavonoids contained in ramie (Boehmeria nivea) root are important resources for officinal use. The plant seedling can grow optimally and develop an easy-harvesting adventitious root system in a hydroponic system. This research was carried out to unravel the regulation mechanism of flavonoid biosynthesis under hydroponic culturing condition, which hitherto remained unknown. Root samples of the ramie plants grown in soil and hydroponic conditions at different developmental stages were collected and pooled for transcriptome sequencing, and the expression levels of flavonoid biosynthesis-related genes and total flavonoid content were quantified. A total of 43,541 unigenes were finally annotated and the expression of 9 key unigenes were confirmed by qRT-PCR. The flavonoid biosynthesis-related gene BnF3H was significantly up-regulated in the roots of hydroponic ramie, and higher total flavonoids content in the hydroponic ramie roots was also observed. The large dataset of transcripts and unigenes provided abundant genetic information for identifying flavonoid biosynthesis genes in ramie roots. Our results suggest flavonoid biosynthesis in ramie roots is positively affected in aquatic environment, and hydroponic culturing could have a potential for enhancing total flavonoids content in ramie roots.

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