Molecular dissection of Secale africanum chromosome 6R(afr) in wheat enabled localization of genes for resistance to powdery mildew and stripe rust

文献类型： 外文期刊

第一作者： Li, Guangrong

作者： Li, Guangrong;Tang, Lingrong;Yin, Yan;Zhang, Ahui;Yu, Zhihui;Yang, Zujun;Yang, Ennian;Tang, Zongxiang;Fu, Shulan

作者机构：

关键词： Adult plant resistance; Powdery mildew; Secale africanum; Stripe rust; Wheat

期刊名称：BMC PLANT BIOLOGY （ 影响因子：4.215； 五年影响因子：4.96 ）

ISSN： 1471-2229

年卷期： 2020 年 20 卷 1 期

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收录情况： SCI

摘要： Background Introgression of chromatin from Secale species into common wheat has for decades been a successful strategy for controlling the wheat diseases. The wild Secale species, Secale africanum Stapf., is a valuable source for resistance to foliar disease of wheat. A wheat-S. africanum chromosome 6R(afr) substitution line displayed resistance to both powdery mildew and stripe rust at the adult-plant stage. Results Wheat-S. africanum chromosome 6R(afr) deletion and translocation lines were produced and identified by sequential non-denaturing fluorescence in situ hybridization (ND-FISH) using multiple Oligo-based probes. Different ND-FISH patterns were observed between S. cereale 6R and S. africanum 6R(afr). With reference to the physical map of the draft genome sequence of rye inbred line Lo7, a comprehensive PCR marker analysis indicated that insertions and deletions had occurred by random exchange between chromosomes 6R and 6R(afr). A survey of the wheat- S. africanum 6R(afr) lines for disease resistance indicated that a powdery mildew resistance gene(s) was present on the long arm of 6R(afr) at FL0.85-1.00, and that a stripe rust resistance gene(s) was located in the terminal region of 6R(afr)S at FL0.95-1.00. The wheat-S. africanum 6R(afr) introgression lines also displayed superior agronomic traits, indicating that the chromosome 6R(afr) may have little linkage drag in the wheat background. Conclusions The combination of molecular and cytogenetic methods allowed to precisely identify the chromosome rearrangements in wheat- S. africanum 6R(afr) substitution, deletion and translocation lines, and compare the structural difference between chromosomes 6R and 6R(afr). The wheat- S. africanum 6R(afr) lines containing gene(s) for powdery mildew and stripe rust resistance could be used as novel germplasm for wheat breeding by chromosome engineering.

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