Genome-Wide Detection of Key Genes and Epigenetic Markers for Chicken Fatty Liver

文献类型： 外文期刊

第一作者： Tan, Xiaodong

作者： Tan, Xiaodong;Liu, Ranran;Xing, Siyuan;Zhang, Yonghong;Li, Qinghe;Zheng, Maiqing;Zhao, Guiping;Wen, Jie;Tan, Xiaodong;Liu, Ranran;Xing, Siyuan;Li, Qinghe;Zheng, Maiqing;Zhao, Guiping;Wen, Jie;Xing, Siyuan

作者机构：

关键词： chicken; fatty liver; lipid metabolism; epigenetics; DNA methylation; long noncoding RNA

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

ISSN：

年卷期： 2020 年 21 卷 5 期

页码：

收录情况： SCI

摘要： Chickens are one of the most important sources of meat worldwide, and the occurrence of fatty liver syndrome (FLS) is closely related to production efficiency. However, the potential mechanism of FLS remains poorly understood. An integrated analysis of data from whole-genome bisulfite sequencing and long noncoding RNA (lncRNA) sequencing was conducted. A total of 1177 differentially expressed genes (DEGs) and 1442 differentially methylated genes (DMGs) were found. There were 72% of 83 lipid- and glucose-related genes upregulated; 81% of 150 immune-related genes were downregulated in fatty livers. Part of those genes was within differentially methylated regions (DMRs). Besides, sixty-seven lncRNAs were identified differentially expressed and divided into 13 clusters based on their expression pattern. Some lipid- and glucose-related lncRNAs (e.g., LNC_006756, LNC_012355, and LNC_005024) and immune-related lncRNAs (e.g., LNC_010111, LNC_010862, and LNC_001272) were found through a co-expression network and functional annotation. From the expression and epigenetic profiles, 23 target genes (e.g., HAO1, ABCD3, and BLMH) were found to be hub genes that were regulated by both methylation and lncRNAs. We have provided comprehensive epigenetic and transcriptomic profiles on FLS in chicken, and the identification of key genes and epigenetic markers will expand our understanding of the molecular mechanism of chicken FLS.

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