Effects of an Escherichia coli exopolysaccharide on human and mouse gut microbiota in vitro

文献类型： 外文期刊

第一作者： Li, Baiyuan

作者： Li, Baiyuan;Chen, Huahai;Cao, Linyan;Hu, Yunfei;Chen, Dan;Yin, Yeshi;Yin, Yeshi

作者机构：

关键词： EPS-RB; Gut microbiota; High-throughput sequencing; Short-chain fatty acid

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）

ISSN： 0141-8130

年卷期： 2020 年 150 卷

页码：

收录情况： SCI

摘要： In this study, an exopolysaccharide (EPS) named EPS-RB was produced when the gene cluster ycjD-fabl-yciW-rnb were overexpressed in E. coli. Monosaccharide composition analysis revealed that EPS-RB is a novel EPS that consisted of L-fucose, L-arabinose, D-galactose/N-acetyl glucosamine, D-glucose, D-xylose, D-ribose, and D-glucuronic add, and their molecular ratio was approximately 80:3:53:69:1:2:64. The content of carbohydrates, protein, and uronic adds in EPS-RB was 90.35 +/- 135%, 2.62 +/- 0.05% and 8.16 +/- 1.00%, respectively. The interaction between EPS-RB and gut microbiota was investigated using an in vitro batch fermentation system. The results showed that -96% of EPS-RB can be degraded by human fecal microbiota after 72 h fermentation, but few can be degraded by mouse cecal microbiota. Furthermore, high-throughput sequencing showed that EPS-RB regulates the human gut microbiota. The genera Collinsella, Butyricimonas, and Hafnia were enriched in group VIR (EPS-RB as a carbon source) when compared with group VI (no carbon source) and VIS (starch as a carbon source). Short-chain fatty acids (SCFAs) production analysis showed that their concentration was significantly higher in group VIR than groups VI and VIS after 72 h fermentation. In summary, an EPS-RB in E. coli was isolated and its regulatory function on gut microbiota was analyzed. (C) 2019 Elsevier B.V. All rights reserved.

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