Comparative Proteomics Analysis Reveals New Features of the Oxidative Stress Response in the Polyextremophilic Bacterium Deinococcus radiodurans

文献类型： 外文期刊

第一作者： Gao, Lihua

作者： Gao, Lihua;Zhou, Zhengfu;Chen, Xiaonan;Zhang, Wei;Lin, Min;Chen, Ming

作者机构：

关键词： Deinococcus radiodurans; oxidative stress response; proteome; antioxidant system

期刊名称：MICROORGANISMS （ 影响因子：4.128； ）

ISSN：

年卷期： 2020 年 8 卷 3 期

页码：

收录情况： SCI

摘要： Deinococcus radiodurans is known for its extreme resistance to ionizing radiation, oxidative stress, and other DNA-damaging agents. The robustness of this bacterium primarily originates from its strong oxidative resistance mechanisms. Hundreds of genes have been demonstrated to contribute to oxidative resistance in D. radiodurans; however, the antioxidant mechanisms have not been fully characterized. In this study, comparative proteomics analysis of D. radiodurans grown under normal and oxidative stress conditions was conducted using label-free quantitative proteomics. The abundances of 852 of 1700 proteins were found to significantly differ between the two groups. These differential proteins are mainly associated with translation, DNA repair and recombination, response to stresses, transcription, and cell wall organization. Highly upregulated expression was observed for ribosomal proteins such as RplB, Rpsl, RpsR, DNA damage response proteins (DdrA, DdrB), DNA repair proteins (RecN, RecA), and transcriptional regulators (members of TetR, AsnC, and GntR families, DdrI). The functional analysis of proteins in response to oxidative stress is discussed in detail. This study reveals the global protein expression profile of D. radiodurans in response to oxidative stress and provides new insights into the regulatory mechanism of oxidative resistance in D. radiodurans.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序