Complete genome sequence of Aphid lethal paralysis virus from metagenomic analysis of Cestrum elegans small RNAs
文献类型： 外文期刊
第一作者： Roumi, Vahid
作者： Roumi, Vahid;Caglayan, Kadriye;Gazel, Mona;Li, Shifang
作者机构：
关键词： Cestrum elegans; Small RNA; NGS; ALPV
期刊名称：GENE REPORTS （ 影响因子：1.3； 五年影响因子：1.1 ）
ISSN：
年卷期： 2020 年 18 卷
页码：
收录情况： SCI
摘要： Passive "vector" plants can harbor insect viruses without supporting their replication and may have epidemiological consequences. We report complete sequence of Aphid lethal paralysis virus (ALPV, Cripavirus, Dicistroviridae) from small RNA sequencing of a new passive "vector" plant, Cestrum elegans in Turkey using de novo assembly and annotating of contigs. Its genome is 9837 nt in length which encodes for 2 ORFs (including an intergenic untranslated region (IGR) of 196 nt) flanked by a 525 nt 5' UTR and a 596 nt 3' UTR. Multiple alignment and phylogenetic analysis showed that the isolate shares highest identity with ALPV-An (JX480861) isolated from oleander aphid, Aphis nerii and was placed into the same Glade with other isolates of passive "vector" plants comprising cucumber and maize isolates.
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