Effects of leptin on fatty acid metabolism in the liver and reproductive function of tongue sole (Cynoglossus semilaevis) in vivo

文献类型： 外文期刊

第一作者： Cai, Xin

作者： Cai, Xin;Li, Yuting;Meng, Zhaojun;Cui, Aijun;Jiang, Yan;Xu, Yongjiang;Meng, Zhaojun

作者机构：

关键词： Leptin; Tongue sole; Lipid metabolism; Regulatory mechanism; Reproductive function

期刊名称：AQUACULTURE （ 影响因子：3.9； 五年影响因子：4.4 ）

ISSN： 0044-8486

年卷期： 2025 年 600 卷

页码：

收录情况： SCI

摘要： To explore the regulatory effect of exogenous recombinant leptin protein (rlepA/rlepB) on fatty acid metabolism and reproductive function in vivo, normal saline (control group), 50 mu g/kg (rlepA, rlepB), 150 mu g/kg (rlepA, rlepB) were injected intraperitoneally into 1-year-old female tongue sole (weighted about 169 g) for four consecutive weeks. Each group had three replicates, with six fish in each replicate. As a result, the low concentrate rlepB group significantly reduced the plumpness, liver acetyl-CoA and muscle crude fat content of fish in the experimental group, while other groups had no significant effect compared with the control group. The transcriptome analysis showed that compared with the control group, KEGG results of each experimental group were enriched in steroid biosynthesis pathway and endocrine resistance. The results of metabolome showed that leptin in all experimental groups significantly decreased the content of saturated fatty acids, and significantly increased the content of unsaturated fatty acids such as docosahexaenoic acid (DHA, C22:6n3), docosapentaenoic acid (DPA, C22:5n3) and arachidonic acid (ARA, C20:4n6). Based on the joint analysis, high concentration rlepA upregulated the expression of pla2 and acot gene sets, which enriched in linoleic acid metabolome and promoted the synthesis of unsaturated fatty acids. Finally, the expression of gnrh3 mRNA in the brain and fsh beta mRNA in the pituitary were upregulated in the rlepB-lc group, as well as the expression of gnrh3 gene and kiss1 gene in the low concentrate rlepA group. Through the joint analysis of transcriptome and metabolome, as well as RT-qPCR, we proposed the possible mechanism of rlepA and rlepB in regulating liver fatty acid metabolism and the possible role in reproduction-related, and analyzed the functional transduction pathway, providing a new idea for the study of leptin on lipid metabolism and reproductive function regulation mechanism of tongue sole. In the future, our research will focus on the regulation of fatty acid metabolism by leptin to achieve the control of reproductive function, which is also an innovative hint for the control of precocious puberty in fish.

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