文献类型： 外文期刊
第一作者： Yu, Dali
作者： Yu, Dali;Liu, Ying;Yu, Dali;Cai, Hongying;Huang, Wanqiu;Yang, Peilong;Wu, Huijun
作者机构：
关键词： Bacterial laccase; Metagenomics; Straw-amended soil; Profile hidden Markov models
期刊名称：PEERJ （ 影响因子：2.4； 五年影响因子：2.7 ）
ISSN： 2167-8359
年卷期： 2025 年 13 卷
页码：
收录情况： SCI
摘要： Background. Bacterial laccases play a crucial role in the degradation of lignin and the turnover of soil organic matter. Their advantageous properties make them highly suitable for a wide range of industrial applications. However, the limited identification of these potential enzymes has impeded their full utilization. The straw-amended soil provides materials for the development of bacterial laccases. Methods. Metagenomic sequencing of a straw-amended soil was conducted to explore novel bacterial laccases. The putative bacterial laccases were then screened using profile hidden Markov models for further analysis. The most abundant gene, lacs1, was heterologously expressed in Escherichia coli and the recombinant laccase was purified for enzymatic characterization. Results. A total of 322 putative bacterial laccases were identified in the straw-amended soil. Among them, 45 sequences had less than 30% identity to any entries in the Carbohydrate-Active Enzyme database and only 4.66% were more than 75% similar to proteins in the NCBI environmental database, exhibiting their novelty. These enzymes were found across various bacterial orders, demonstrating substantial diversity. Phylogenetic analysis revealed a number of the bacterial laccase sequences clustered with homologs characterized by favorable enzymatic properties. Five full-length representative bacterial laccase genes were obtained by modified thermal asymmetric interlaced PCR. The laccase activity of lacS1 was validated. It was a mesophilic enzyme with alkaline stability and halotolerance, indicating its promise for industrial applications. Implications. These findings highlight novel bacterial laccase resources with potential for industrial applications and enzyme engineering.
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