Lutein-stevioside nanoparticle attenuates H2O2-induced oxidative damage in ARPE cells

文献类型： 外文期刊

第一作者： Dai, Zhuqing

作者： Dai, Zhuqing;Nie, Meimei;Chen, Ye;Song, Jiangfeng;Xu, Yayuan;Zhang, Zhongyuan;Li, Dajing;Zhang, Zhongyuan;Zhang, Guodong;Zhang, Xing;Yan, Shumo

作者机构：

关键词： Lutein; Stevioside; Antioxidant; Human retinal pigment epithelial cell; Mechanism

期刊名称：FOOD SCIENCE AND HUMAN WELLNESS （ 影响因子：7.0； 五年影响因子：8.3 ）

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年卷期： 2024 年 13 卷 3 期

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收录情况： SCI

摘要： In order to improve the bioavailability of lutein (LUT), a novel lutein-stevioside nanoparticle (LUT-STE) were prepared previously, but the information about LUT-STE on protecting of eye health was limited. This study investigated the effect of LUT-STE on antioxidant activity of H2O2-induced human retinal pigment epithelial (ARPE) cells. LUT and LUT-STE (final concentration of 5 mu g/mL) significantly enhanced cell viability from (74.84 +/- 5.10)% to (81.92 +/- 10.01)% (LUT) and (89.33 +/- 4.34)% (LUT-STE), and inhibited the cell apoptosis (P < 0.05). After pretreatment with LUT-STE in ARPE cells, the levels of superoxide dismutase (SOD), catalase (CAT) and glutathion peroxidase (GSH-Px) in ARPE cells were significantly increased (P < 0.05), the contents of reactive oxygen species (ROS) and malondialdehyde (MDA) were decreased. In addition, the vascular endothelial growth factor (VEGF) levels were inhibited by 13.61% and 17.39%, respectively, pretreatment with LUT and LUT-STE. Western blotting results showed that the pretreatment with LUT-STE inhibited the expression of caspase-9 and caspase-3 and up-regulated Bcl-2/Bax pathway to inhibit H2O2-induced apoptosis. In summary, the novel delivery LUT-STE had more pronounced inhibitory effect on H2O2-induced damage in human ARPE cells. (c) 2024 Beijing Academy of Food Sciences. Publishing services by Tsinghua University Press. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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