Construction of a recombinant BHV-1 expressing the VP1 gene of foot and mouth disease virus and its immunogenicity in a rabbit model

文献类型: 外文期刊

第一作者: Ren, Xian-Gang

作者: Ren, Xian-Gang;Xue, Fei;Zhu, Yuan-Mao;Tong, Guang-Zhi;Wang, Yan-Hui;Feng, Jun-Ke;Shi, Hong-Fei;Gao, Yu-Ran

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关键词: Foot-and-mouth disease virus;Immune response in rabbits;Recombinant bovine herpesvirus type 1

期刊名称:BIOTECHNOLOGY LETTERS ( 影响因子:2.461; 五年影响因子:2.457 )

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收录情况: SCI

摘要: Foot-and-mouth disease (FMD) and infectious bovine rhinotracheitis (IBR) are two important infectious diseases of cattle. Using bovine herpesvirus type 1 (BHV-1) as a gene delivery vector for development of live-viral vaccines has gained widespread interest. In this study, a recombinant BHV-1 was constructed by inserting the synthetic FMDV (O/China/99) VP1 gene in the the gE locus of BHV-1 genome under the control of immediately early gene promoter of human cytomegalovirus (phIE CMV) and bovine growth hormone polyadenylation (BGH polyA) signal. After homologous recombination and plaque purification, a recombinant virus named BHV-1/gE/VP1 was acquired and identified. The immunogenicity was confirmed in a rabbit model by virus neutralization test and enzyme-linked immunosorbent assay (ELISA). The result indicated that the BHV-1/gE/VP1 has the potential for being developed as a bivalent vaccine for FMD and IBR.

分类号: Q7

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