Construction of a recombinant BHV-1 expressing the VP1 gene of foot and mouth disease virus and its immunogenicity in a rabbit model
文献类型: 外文期刊
第一作者: Ren, Xian-Gang
作者: Ren, Xian-Gang;Xue, Fei;Zhu, Yuan-Mao;Tong, Guang-Zhi;Wang, Yan-Hui;Feng, Jun-Ke;Shi, Hong-Fei;Gao, Yu-Ran
作者机构:
关键词: Foot-and-mouth disease virus;Immune response in rabbits;Recombinant bovine herpesvirus type 1
期刊名称:BIOTECHNOLOGY LETTERS ( 影响因子:2.461; 五年影响因子:2.457 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Foot-and-mouth disease (FMD) and infectious bovine rhinotracheitis (IBR) are two important infectious diseases of cattle. Using bovine herpesvirus type 1 (BHV-1) as a gene delivery vector for development of live-viral vaccines has gained widespread interest. In this study, a recombinant BHV-1 was constructed by inserting the synthetic FMDV (O/China/99) VP1 gene in the the gE locus of BHV-1 genome under the control of immediately early gene promoter of human cytomegalovirus (phIE CMV) and bovine growth hormone polyadenylation (BGH polyA) signal. After homologous recombination and plaque purification, a recombinant virus named BHV-1/gE/VP1 was acquired and identified. The immunogenicity was confirmed in a rabbit model by virus neutralization test and enzyme-linked immunosorbent assay (ELISA). The result indicated that the BHV-1/gE/VP1 has the potential for being developed as a bivalent vaccine for FMD and IBR.
分类号: Q7
- 相关文献
作者其他论文 更多>>
-
3D hot spot construction on the hydrophobic interface with SERS tags for quantitative detection of pesticide residues on food surface
作者:Wang, Yan-Hui;Huang, Chen;Wu, Xiao;Liu, Xiao-Feng;Wang, An;Jin, Shangzhong;Zhang, Fan-Li;You, En-Ming;Liu, Sheng-Hong
关键词:Surface-enhanced Raman scattering; 3D hot spots; Hydrophobic interface; Pesticide residues; Quantitative analysis
-
Characterization of acetolactate synthase genes and resistance mechanisms of multiple herbicide resistant Lolium multiflorum
作者:Xu, Hongle;Cheng, Jingping;Leng, Qiuli;Su, Wangcang;Sun, Lanlan;Xue, Fei;Wu, Renhai;Xu, Hongle;Cheng, Jingping;Leng, Qiuli;Su, Wangcang;Sun, Lanlan;Xue, Fei;Wu, Renhai;Cao, Ran;Han, Yun
关键词:Lolium multiflorum; Acetolactate synthase; Imazamox; Multiple resistance; P450; GST
-
Engineered Plasmonic multi-hot spots on magnetic Nanospheres for quantitative detection of pesticide residues on food surfaces via SERS tagging analysis
作者:Wan, Ming-Jie;Wu, Yu-Ting;Wang, Yan-Hui;Wang, An;Jin, Shangzhong;Zhang, Fan-Li;Wu, Xiao-Hang;Zhang, Yue-Jiao;You, En-Ming;Liu, Sheng-Hong;Chen, Jin-Chao
关键词:Surface-enhanced Raman scattering; Magnetical plasmonic nanostructure; Pesticide residues detection; Raman internal standard; Quantitative analysis
-
Genetic, metabolomic and transcriptomic analyses of the cotton yellow anther trait
作者:Liang, Qian;Feng, Xiaokang;Wang, Xuefeng;Ma, Xiaohu;Liang, Rui;Liu, Feng;Zhang, Xinyu;Sun, Jie;Xue, Fei;Hu, Daowu;He, Shoupu;Jin, Yanlong;Zhu, Qian-Hao;Zhu, Huaguo
关键词:Carotenoid biosynthesis; Flavonoid biosynthesis; PSY; Wild cotton; Yellow anther
-
Organic Porous Materials with Temperature Responsive Coatings for Mesosulfuron-Methyl Delivery
作者:Su, Wangcang;Niu, Yujia;Chen, Wenzhe;Sun, Lanlan;Xue, Fei;Xu, Hongle;Wu, Renhai;Yang, Aiguo;Zhao, Shirui
关键词:temperature-controlled release; mesosulfuron-methyl;
Aegilops tauschii ; safety; efficacy -
pseudo-GhFAD2-1 Is a lncRNA Involved in Regulating Cottonseed Oleic and Linoleic Acid Ratios and Seed Size in Gossypium hirsutum
作者:Chen, Haihong;Li, Yanjun;Xue, Fei;Sun, Jie;Liu, Feng;Xiong, Xianpeng;Liu, Xuan;Zhu, Qian-Hao
关键词:cottonseed oil; GhFAD2-1; histone deacetylase 1; long non-coding RNA; seed size
-
Identification and characterization of linear epitopes of monoclonal antibodies against the capsid proteins of small ruminant lentiviruses
作者:Ma, Xiaohua;Gao, Min;Zhang, Xiangmin;Ma, Weiwei;Xue, Fei;Wang, Xue-Feng;Wang, Xiaojun;Wang, Xiaojun
关键词:small ruminant lentiviruses (SRLVs); maedi-visna virus (MVV); caprine arthritis encephalitis virus (CAEV); monoclonal antibodies (mAbs); capsid protein