Isolation of genes expressed during compatible interactions between powdery mildew (Blumeria graminis) and wheat
文献类型: 外文期刊
第一作者: Zheng, Li
作者: Zheng, Li;Huang, Junbin;Zheng, Li;Yu, Dazhao
作者机构:
关键词: amino acid sequence;expression pattern;nucleotide sequence;GenBank accession number;compatible interaction
期刊名称:PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY ( 影响因子:2.747; 五年影响因子:2.388 )
ISSN:
年卷期:
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收录情况: SCI
摘要: Powdery mildew of wheat is an important disease caused by the obligately biotrophic fungus Blumeria graminis. In compatible interactions, the powdery mildew fungus undergoes a series of developmental stages to form haustoria within host cells through which nutrients are obtained. In this study, we utilized the cDNA-AFLP technique to isolate wheat genes expressed at 5 days' post-inoculation. The expression patterns of several sequences identified in the cDNA-AFLP pro. ling were further investigated by Northern hybridization and RT-PCR analyses. Genes with sequence similarity to GenBank accessions AAR91119, P20076, BT009372, ABA99697, BAD81963 or AAO72574 showed greater expression in susceptible rather than resistant or mock-inoculated leaves. In addition, several sequences with similarity to CAD27894, XM_466672, AAT79487 or AAM64566 were expressed only in the compatible interactions. Possible involvement of these genes in susceptibility of host wheat upon powdery mildew infection is further discussed.
分类号: S432.1
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