Multi-omics analysis reveals the evolutionary origin of diterpenoid alkaloid biosynthesis pathways in Aconitum

文献类型： 外文期刊

第一作者： Zhao, Dake

作者： Zhao, Dake;Dong, Ding;Li, Zonghang;Mou, Zongmin;Chen, Suiyun;Zhang, Ya;Ruan, Jue;Ren, Huanxing;Cun, Yupeng;Ren, Huanxing;Cun, Yupeng;Shi, Yana;Huang, Luqi;Cui, Guanghong;Huang, Luqi;Shen, Yong;Kennelly, Edward J.;Kennelly, Edward J.;Yu, Diqiu

作者机构：

关键词： Aconitum vilmorinianum; acute toxicity; biosynthetic pathway; diterpenoid alkaloid; evolutionary origin; genome assembly

期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 影响因子：11.4； 五年影响因子：10.1 ）

ISSN： 1672-9072

年卷期： 2023 年 65 卷 10 期

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收录情况： SCI

摘要： Diterpenoid alkaloids (DAs) have been often utilized in clinical practice due to their analgesic and anti-inflammatory properties. Natural DAs are prevalent in the family Ranunculaceae, notably in the Aconitum genus. Nevertheless, the evolutionary origin of the biosynthesis pathway responsible for DA production remains unknown. In this study, we successfully assembled a high-quality, pseudochromosome-level genome of the DA-rich species Aconitum vilmorinianum (A. vilmorinianum) (5.76 Gb). An A. vilmorinianum-specific whole-genome duplication event was discovered using comparative genomic analysis, which may aid in the evolution of the DA biosynthesis pathway. We identified several genes involved in DA biosynthesis via integrated genomic, transcriptomic, and metabolomic analyses. These genes included enzymes encoding target ent-kaurene oxidases and aminotransferases, which facilitated the activation of diterpenes and insertion of nitrogen atoms into diterpene skeletons, thereby mediating the transformation of diterpenes into DAs. The divergence periods of these genes in A. vilmorinianum were further assessed, and it was shown that two major types of genes were involved in the establishment of the DA biosynthesis pathway. Our integrated analysis offers fresh insights into the evolutionary origin of DAs in A. vilmorinianum as well as suggestions for engineering the biosynthetic pathways to obtain desired DAs.

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