Fluorescence biosensor to detect microRNAs via integrating DNA hairpins transition mediated strand displacement amplification with primer exchange reaction

文献类型： 外文期刊

第一作者： Liu, Xiaoyu

作者： Liu, Xiaoyu;Li, Zehong;Bu, Shengjun;Zhou, Hongyu;Xu, Yao;Hao, Zhuo;Wan, Jiayu;Bu, Shengjun

作者机构：

关键词： miRNA-21; DNA hairpins transition; Strand displacement amplification; Primer exchange reaction; Detection

期刊名称：BIOORGANIC & MEDICINAL CHEMISTRY LETTERS （ 影响因子：2.7； 五年影响因子：2.6 ）

ISSN： 0960-894X

年卷期： 2024 年 106 卷

页码：

收录情况： SCI

摘要： Herein, we constructed a fluorescence biosensor for the ultra-sensitive analysis of microRNAs (miRNAs) by combining DNA hairpins transition triggered strand displacement amplification (DHT-SDA) with primer exchange reaction (PER). Target miRNA initiated DHT-SDA to facilitate the generation of multiple single-stranded DNA (ssDNA) as PER primer, which was extended into a long ssDNA. The biosensor is successfully utilized in detecting miRNAs with high sensitivity (limit of detection for miRNA-21 was 58 fM) and a good linear relationship between 100 nM and 100 fM. By simply changing the DNA hairpin sequence, the constructed biosensor can be extended to analyze another miRNAs. Moreover, the biosensor has the feasibility of detecting miRNAs in real samples with satisfactory accuracy and reliability. Therefore, the fluorescent biosensor has great application potential in clinical diagnosis.

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