Full-length agave transcriptome reveals candidate glycosyltransferase genes involved in hemicellulose biosynthesis

文献类型： 外文期刊

第一作者： Huang, Xing

作者： Huang, Xing;Tan, Shibei;Wu, Weihuai;Hu, Xiaoli;Xie, Zhouli;Liu, Qingqing;Qin, Xu;Chen, Tao;Saud, Shah;Nawaz, Taufiq;Fahad, Shah;El-Kahtany, Khaled;Fahad, Shah;Yi, Kexian;Yi, Kexian;Yi, Kexian

作者机构：

关键词： Agave; Full-length transcriptome; Glycosyltransferase gene; Hemicellulose biosynthesis; Leaf development; Fungus infection

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：7.7； 五年影响因子：7.7 ）

ISSN： 0141-8130

年卷期： 2024 年 274 卷

页码：

收录情况： SCI

摘要： Agave species are typical crassulacean acid metabolism (CAM) plants commonly cultivated to produce beverages, fibers, and medicines. To date, few studies have examined hemicellulose biosynthesis in Agave H11648, which is the primary cultivar used for fiber production. We conducted PacBio sequencing to obtain full-length transcriptome of five agave tissues: leaves, shoots, roots, flowers, and fruits. A total of 41,807 genes were generated, with a mean length of 2394 bp and an annotation rate of 97.12 % using public databases. We identified 42 glycosyltransferase genes related to hemicellulose biosynthesis, including mixed-linkage glucan (1), glucomannan (5), xyloglucan (16), and xylan (20). Their expression patterns were examined during leaf development and fungal infection, together with hemicellulose content. The results revealed four candidate glycosyltransferase genes involved in xyloglucan and xylan biosynthesis, including glucan synthase (CSLC), xylosyl transferase (XXT), xylan glucuronyltransferase (GUX), and xylan alpha-1,3-arabinosyltransferase (XAT). These genes can be potential targets for manipulating xyloglucan and xylan traits in agaves, and can also be used as candidate enzymatic tools for enzyme engineering. We have provided the first full-length transcriptome of agave, which will be a useful resource for gene identification and characterization in agave species. We also elucidated the hemicellulose biosynthesis machinery, which will benefit future studies on hemicellulose traits in agave.

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