Isolation of sex-specific AFLP markers in Spotted Halibut (Verasper variegatus)
文献类型: 外文期刊
第一作者: Ma, Hongyu
作者: Ma, Hongyu;Chen, Songlin;Yang, Jingfeng;Ji, Xiangshan;Chen, Siqing;Tian, Yongsheng;Bi, Jinzhen;Ma, Hongyu
作者机构:
关键词: AFLP;Female;specific markers;Verasper variegatus;Genetic sex identification
期刊名称:ENVIRONMENTAL BIOLOGY OF FISHES ( 影响因子:1.844; 五年影响因子:1.839 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Spotted Halibut (Verasper variegatus) is a commercially important marine fish species. In the present study, to isolate sex-specific markers in Spotted Halibut, we screened the genomes of Spotted Halibut by AFLP technique with 64 different primer combinations. Two primer combinations, MseI-CAG/EcoRI-ACC and MseI-CAT/EcoRI-AGG, produced a female-specific fragment in all females (n=88) and in no males (n=60, except 3 individuals), respectively. Both fragments were excised from the gel, cloned and characterized. The first fragment (named VevaF533) was 533 bp long, while the length of the second one (named VevaF218) was 218 bp. The two sequences showed no similarity to each other, and to the known sequences existing in the GenBank database using BLASTn. Cross-species amplification showed that the marker VevaF218 is a species-specific marker which is present in Spotted Halibut females but absent in Barfin Flounder (Verasper moseri). So this marker could be used for discriminating unambiguously between Spotted Halibut females and Barfin Flounder. Examination of the patterns of inheritance of VevaF218 in an interspecific hybrid family (V. variegatus cV. moseri ) showed a female-specific pattern of inheritance from mother to daughter, implying that the marker VevaF218 is located on the female sex chromosome. This study provides a reliable AFLP-based genetic sexing of Spotted Halibut that could be useful for genetic mapping of the sex chromosomes and identification of sex-linked genes.
分类号: S9
- 相关文献
作者其他论文 更多>>
-
Bile acids help maintain immune homeostasis and enhance antioxidant capacity upon Vibrio infection in leopard coral grouper
作者:Zhang, Junwei;Yang, Bowen;Shi, Meng;Chen, Songlin;Zhou, Qian;Zhang, Junwei;Zhang, Jiquan;Wang, Shengpeng
关键词:Plectropomus leopardus; BAs; Vibrio harveyi infection; Transcriptomics; Metabolomics
-
A QTL associated with vibriosis resistance highlights the significant role of jam2a gene in tongue sole (Cynoglossus semilaevis)
作者:Li, Yangzhen;Chi, Yong;Yan, Xu;Hu, Yuanri;Chen, Songlin;Li, Yangzhen;Chen, Songlin;Li, Zhongming;Jia, Lei
关键词:Genome-wide association; Quantitative trait locus; Transcriptome analysis; Heritability; Immune response
-
Orchestrated immune responses to Mycobacterium marinum natural infection in tongue sole ( Cynoglossus semilaevis)
作者:Li, Yangzhen;Yan, Xu;Chi, Yong;Hu, Yuanri;Wang, Jialin;Chen, Songlin;Li, Yangzhen;Chen, Songlin;Song, Limin;Robeldo, Diego;Mukiibi, Robert;Robeldo, Diego;Mukiibi, Robert;Robeldo, Diego;Mukiibi, Robert
关键词:Mycobacteriosis; Bacterial infection; RNA-Seq; Transcriptome analysis; Disease resistance
-
Chrome-Free Tanning Process of Thamnaconus septentrionalis Skin
作者:Pan, Luying;Yang, Yun;Kong, Qing;Pan, Luying;Yang, Yun;Bian, Li;Chen, Siqing;Kong, Qing
关键词:Thamnaconus septentrionalis; Fish skin waste utilization; Chrome-free tanning; Fish leather; Application
-
Genome-wide association study and transcriptome analysis provide the SNPs and molecular insights into the visceral nodular disease tolerance in half-smooth tongue sole (Cynoglossus semilaevis)
作者:Chang, Mengyang;Shi, Kunpeng;Xia, Jinqi;Zhang, Ruixue;Li, Yishuai;Zang, Shaoqing;Tan, Suxu;Wang, Wenwen;Sha, Zhenxia;Chen, Songlin;Sha, Zhenxia;Chen, Songlin
关键词:Cynoglossus semilaevis; Visceral nodular disease; GWAS; RNA-seq; Disease tolerance; Multi omics
-
A newly developed 20 K SNP array reveals QTLs for disease resistance to Cryptocaryon irritans in tiger pufferfish (Takifugu rubripes)
作者:Li, Yangzhen;Fraslin, Clemence;Chi, Yong;Hu, Yuanri;Wang, Jialin;Chen, Songlin;Li, Yangzhen;Mukiibi, Robert;Robledo, Diego;Li, Yangzhen;Li, Weidong;Li, Weiguo;Hu, Yuanri;Wang, Jialin;Chen, Songlin;Houston, Ross D.;Robledo, Diego
关键词:SNP array; Heritability; Genomic prediction; GWAS; QTL; Selective breeding
-
SHP-1 interacts with NFκB1 to inhibit its phosphorylation and nuclear translocation to exert its anti-bacterial function
作者:Wang, Ningning;Tan, Suxu;Wang, Muyuan;Liu, Hongning;Han, Sen;Wu, Zhendong;Ma, Jie;Chen, Songlin;Sha, Zhenxia;Wang, Ningning;Chen, Songlin;Chen, Songlin;Sha, Zhenxia
关键词:SHP-1; Immune response; Phosphorylation of NF kappa B1; Nuclear translocation