Sequence and Phylogenetic Analysis of SSU rRNA Gene of Five Microsporidia

文献类型： 外文期刊

第一作者： Shen, ZhongYuan

作者： Shen, ZhongYuan;Xu, Li;Dong, ShiNan;Zhu, Feng;Dong, ShiNan;Shen, ZhongYuan;Xu, Li;Zhu, Feng

作者机构：

关键词： Population Genetics: Population Studies;Molecular Genetics: Biochemistry and Molecular Biophysics

期刊名称：CURRENT MICROBIOLOGY （ 影响因子：2.188； 五年影响因子：2.197 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The complete small subunit rRNA (SSU rRNA) gene sequences of five microsporidia including Nosema heliothidis, and four novel microsporidia isolated from Pieris rapae, Phyllobrotica armta, Hemerophila atrilineata, and Bombyx mori, respectively, were obtained by PCR amplification, cloning, and sequencing. Two phylogenetic trees based on SSU rRNA sequences had been constructed by using Neighbor-Joining of Phylip software and UPGMA of MEGA4.0 software. The taxonomic status of four novel microsporidia was determined by analysis of phylogenetic relationship, length, G+C content, identity, and divergence of the SSU rRNA sequences. The results showed that the microsporidia isolated from Pieris rapae, Phyllobrotica armta, and Hemerophila atrilineata have close phylogenetic relationship with the Nosema, while another microsporidium isolated from Bombyx mori is closely related to the Endoreticulatus. So, we temporarily classify three novel species of microsporidia to genus Nosema, as Nosema sp. PR, Nosema sp. PA, Nosema sp. HA. Another is temporarily classified into genus Endoreticulatus, as Endoreticulatus sp. Zhenjiang. The result indicated as well that it is feasible and valuable to elucidate phylogenetic relationships and taxonomic status of microsporidian species by analyzing information from SSU rRNA sequences of microsporidia.

分类号： Q93

  • 相关文献

[1]Cloning and Expression of the Porcine MEF2a Gene. Zhang, Dong-jie,Liu, Di,Yang, Guo-wei,Gao, Zhi-sheng.

[2]Transgene expression of enhanced green fluorescent protein in cloned rabbits generated from in vitro-transfected adult fibroblasts. Li, Shangang,Yin, Chunguang,Xu, Lingyang,Liu, Tao,Li, Hongbin,Du, Lixin,Guo, Yi,Shi, Jianjun,Xing, Fengying,Li, Yao,Chen, Xuejin,Zhang, Chuanshan.

[3]Evaluation of Echinococcus multilocularis tetraspanins as vaccine candidates against primary alveolar echinococcosis. Dang, Zhisheng,Kajino, Kiichi,Nakao, Ryo,Sugimoto, Chihiro,Dang, Zhisheng,Yagi, Kinpei,Kouguchi, Hirokazu,Oku, Yuzaburo,Matsumoto, Jun,Watanabe, Junichi,Matsumoto, Jun,Wakaguri, Hiroyuki,Toyoda, Atsushi,Toyoda, Atsushi.

[4]Molecular mapping of powdery mildew resistance gene PmHNK in winter wheat (Triticum aestivum L.) cultivar Zhoumai 22. Xu, W. G.,Li, C. X.,Hu, L.,Zhang, L.,Zhang, J. Z.,Dong, H. B.,Wang, G. S.,Li, C. X..

[5]A simple artificial microRNA vector based on ath-miR169d precursor from Arabidopsis. Liu, Chong,Zhang, Lan,Luo, Yanzhong,Fan, Yun-Liu,Wang, Lei,Liu, Chong,Sun, Jie,Wang, Ming-Bo.

[6]Construction of Mammalian Cell Expression Vector for pAcGFP-bFLIP(L) Fusion Protein and Its Expression in Follicular Granulosa Cells. Yang, Run Jun,Li, Jun Ya,Zhang, Lu Pei,Gao, Xue,Chen, Jin Bao,Xu, Shang Zhong.

[7]Ri-mediated Transformation of Glycyrrhiza uralensis with a Squalene Synthase Gene (GuSQS1) for Production of Glycyrrhizin. Lu, Hong-Yu,Zhang, Hai-Chao,Gao, Shan-Lin,Liu, Jing-Mei,Yin, Tao.

[8]Molecular Cloning and Expression of cDNA Encoding the Cysteine Proteinase Inhibitor from Upland Cotton. Jiang, Ming-feng,Li, Sheng-wei,Chen, Min,Li, Sheng-wei,Chen, Min,Cai, Ying-fan,Xie, Yong-fang,Li, Biao,Sun, Quan,Jiang, Huai-zhong,Pan, Zheng,Gao, Yun-ling,Yuan, You-Lu,Shi, Yu-zheng.

[9]The development and evaluation of a loop-mediated isothermal amplification (LAMP) method for detection of Babesia spp. infective to sheep and goats in China. Guan, Guiquan,Luo, Jianxun,Liu, Zhijie,Gao, Jinliang,Ma, Miling,Liu, Aihong,Dang, Zhisheng,Liu, Junlong,Ren, Qiaoyun,Jin, Yurong,Yin, Hong,Guan, Guiquan,Chauvin, Alain,Moreau, Emmanuelle,Inoue, Noboru,Thekisoe, Oriel M. M.,Sugimoto, Chihiro.

[10]RGA-ILP, a new type of functional molecular markers in bread wheat. Shang, World,Zhou, Ronghua,Jia, Jizeng,Gao, Lifeng.

[11]Establishment and Characterization of the Fibroblast Line from Silkie Bantam. Li, L. F.,Guan, W. J.,Li, H.,Ma, Y. H..

[12]Identification of Heterodera glycines using PCR with sequence characterised amplified region (SCAR) primers. Peng, Deliang,Ou, Shiqi,Liu, Xuemin,Li, Yu,Moens, Maurice,Moens, Maurice.

[13]Characterization of Five Putative Acyl Carrier Protein (ACP) Isoforms from Developing Seeds of Arachis hypogaea L.. Li, Meng-Jun,Wang, Xing-Jun,Su, Lei,Bi, Yu-Ping,Wan, Shu-Bo.

[14]Characterization of the H5N1 Highly Pathogenic Avian Influenza Virus Derived from Wild Pikas in China. Zhou, Jiyong,Sun, Wenbo,Wang, Junhua,Guo, Junqing,Yin, Wei,Yan, Yan,Jiang, Xuetao,Zhou, Jiyong,Sun, Wenbo,Wang, Junhua,Guo, Junqing,Yin, Wei,Wu, Nanping,Li, Lanjuan,Yan, Yan,Jiang, Xuetao,Zhou, Jiyong,Sun, Wenbo,Wang, Junhua,Guo, Junqing,Yan, Yan,Chen, Hualan,Liao, Ming,Luo, Kaijian,Huang, Yu.

[15]Cyanobacteria MT gene SmtA enhance zinc tolerance in Arabidopsis. Xu, Jing,Hou, Xi-Lin,Xu, Jing,Tian, Yong-Sheng,Peng, Ri-He,Xiong, Ai-Sheng,Zhu, Bo,Yao, Quan-Hong.

[16]SSR based linkage and mapping analysis of C, a yellow cocoon gene in the silkworm, Bombyx mori. Zhao, Yun-Po,Li, Mu-Wang,Xu, An-Ying,Hou, Cheng-Xiang,Guo, Xi-Jie,Zhao, Yun-Po,Guo, Xi-Jie,Li, Ming-Hui,Guo, Qiu-Hong,Huang, Yong-Ping.

[17]'Candidatus Phytoplasma tamaricis', a novel taxon discovered in witches'-broom-diseased salt cedar (Tamarix chinensis Lour.). Zhao, Yan,Sun, Qingrong,Wei, Wei,Davis, Robert E.,Wu, Wei,Liu, Qingzhong,Sun, Qingrong,Liu, Qingzhong.

[18]Constitutive expression of ZmsHSP in Arabidopsis enhances their cytokinin sensitivity. Liu, Yongjun,Zheng, Jun,Wang, Guoying,Cao, Zuping,Jia, Zhiwei,Wang, Meng,Zhao, Jinfeng.

[19]Identification and characterization of inosine 5-monophosphate dehydrogenase in Streptococcus suis type 2. Zhang, Xue-han,He, Kong-wang,Duan, Zhi-tao,Zhou, Jun-ming,Yu, Zheng-yu,Ni, Yan-xiu,Zhang, Xue-han,He, Kong-wang,Duan, Zhi-tao,Zhou, Jun-ming,Yu, Zheng-yu,Ni, Yan-xiu,Zhang, Xue-han,He, Kong-wang,Duan, Zhi-tao,Zhou, Jun-ming,Yu, Zheng-yu,Ni, Yan-xiu,Lu, Cheng-ping.

[20]Development of SNP markers and haplotype analysis of the candidate gene for rhg1, which confers resistance to soybean cyst nematode in soybean. Li, Ying-Hui,Zhang, Chen,Ma, Zulu,Liu, Zhang-Xiong,Nan, Hai-Yang,Chang, Ru-Zhen,Qiu, Li-Juan,Gao, Zhong-Shan,Smulders, Marinus Johannes Maria,Ma, Zulu.

作者其他论文 更多>>

微信小程序