Effect of nutritional and environmental conditions on biofilm formation of avian pathogenic Escherichia coli

文献类型： 外文期刊

第一作者： Hu, Jiangang

作者： Hu, Jiangang;Bao, Yinli;Yin, Huifang;Huang, Cuiqin;Han, Xiangan;Hu, Jiangang;Lv, Xiaolong;Niu, Xiangpeng;Yu, Fangheng;Zuo, Jiakun;Nawaz, Saqib;Zhou, Wen;Jiang, Wei;Chen, Zhaoguo;Han, Xiangan;Hu, Jiangang;Lv, Xiaolong;Niu, Xiangpeng;Yu, Fangheng;Tu, Jian;Qi, Kezong

作者机构：

关键词： avian pathogenic Escherichia coli; biofilm; environment; glucose; nutrient

期刊名称：JOURNAL OF APPLIED MICROBIOLOGY （ 影响因子：4.059； 五年影响因子：4.439 ）

ISSN： 1364-5072

年卷期： 2022 年 132 卷 6 期

页码：

收录情况： SCI

摘要： Aims To study the effects of environmental stress and nutrient conditions on biofilm formation of avian pathogenic Escherichia coli (APEC). Methods and Results The APEC strain DE17 was used to study biofilm formation under various conditions of environmental stress (including different temperatures, pH, metal ions, and antibiotics) and nutrient conditions (Luria-Bertani [LB] and M9 media, with the addition of different carbohydrates, if necessary). The DE17 biofilm formation ability was strongest at 25 degrees C in LB medium. Compared to incubation at 37 degrees C, three biofilm-related genes (csgD, dgcC, and pfs) were significantly upregulated and two genes (flhC and flhD) were downregulated at 25 degrees C, which resulted in decreased motility. However, biofilm formation was strongest in M9 medium supplemented with glucose at 37 degrees C, and the number of live bacteria was the highest as determined by confocal laser scanning microscopy. The bacteria in the biofilm were surrounded by a thick extracellular matrix, and honeycomb-like or rough surfaces were observed by scanning electron microscopy. Moreover, biofilm formation of the DE17 strain was remarkably inhibited under acidic conditions, whereas neutral and alkaline conditions were more suitable for biofilm formation. Biofilm formation was also inhibited at specific concentrations of cations (Na+, K+, Ca2+, and Mg2+) and antibiotics (ampicillin, chloramphenicol, kanamycin, and spectinomycin). The real-time quantitative reverse transcription PCR showed that the transcription levels of biofilm-related genes change under different environmental conditions. Conclusions Nutritional and environmental factors played an important role in DE17 biofilm development. The transcription levels of biofilm-related genes changed under different environmental and nutrient conditions. Significance and Impact of the Study The findings suggest that nutritional and environmental factors play an important role in APEC biofilm development. Depending on the different conditions involved in this study, it can serve as a guide to treating biofilm-related infections and to eliminating biofilms from the environment.

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