The CD2v protein of African swine fever virus inhibits macrophage migration and inflammatory cytokines expression by downregulating EGR1 expression through dampening ERK1/2 activity

文献类型： 外文期刊

第一作者： Zhang, Min

作者： Zhang, Min;Lv, Lilei;Luo, Huaye;Cai, Hongming;Yu, Lingxue;Jiang, Yifeng;Gao, Fei;Tong, Wu;Li, Liwei;Li, Guoxin;Zhou, Yanjun;Tong, Guangzhi;Liu, Changlong;Zhang, Min;Tong, Guangzhi;Cai, Hongming;Yu, Lingxue;Jiang, Yifeng;Gao, Fei;Tong, Wu;Li, Liwei;Li, Guoxin;Zhou, Yanjun;Tong, Guangzhi;Liu, Changlong

作者机构：

关键词： ASFV; CD2v; cell migration; EGR1; ERK1/2; inflammatory cytokine

期刊名称：VETERINARY RESEARCH （ 影响因子：4.4； 五年影响因子：4.3 ）

ISSN： 0928-4249

年卷期： 2023 年 54 卷 1 期

页码：

收录情况： SCI

摘要： African swine fever virus (ASFV) is a highly contagious and deadly virus that leads to high mortality rates in domestic swine populations. Although the envelope protein CD2v of ASFV has been implicated in immunomodulation, the molecular mechanisms underlying CD2v-mediated immunoregulation remain unclear. In this study, we generated a stable CD2v-expressing porcine macrophage (PAM-CD2v) line and investigated the CD2v-dependent transcriptomic landscape using RNA-seq. GO terms enrichment analysis and gene set enrichment analysis revealed that CD2v predominantly affected the organization and assembly process of the extracellular matrix. Wound healing and Transwell assays showed that CD2v inhibited swine macrophage migration. Further investigation revealed a significant decrease in the expression of transcription factor early growth response 1 (EGR1) through inhibiting the activity of extracellular signal-regulated kinase 1 and 2 (ERK1/2). Notably, EGR1 knockout in swine macrophages restricted cell migration, whereas EGR1 overexpression in PAM-CD2v restored the ability of macrophage migration, suggesting that CD2v inhibits swine macrophage motility by downregulating EGR1 expression. Furthermore, we performed chromatin immunoprecipitation and sequencing for EGR1 and the histone mark H3K27 acetylation (H3K27ac), and we found that EGR1 co-localized with the activated histone modification H3K27ac neighboring the transcriptional start sites. Further analysis indicated that EGR1 and H3K27ac co-occupy the promoter regions of cell locomotion-related genes. Finally, by treating various derivatives of swine macrophages with lipopolysaccharides, we showed that depletion of EGR1 decreased the expression of inflammatory cytokines including TNF alpha, IL1 alpha, IL1 beta, IL6, and IL8, which play essential roles in inflammation and host immune response. Collectively, our results provide new insights into the immunomodulatory mechanism of ASFV CD2v.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序