A novel approach obtaining intron-containing hairpin RNA constructs

文献类型: 外文期刊

第一作者: Cai, Ping-Zhong

作者: Cai, Ping-Zhong;Wen, Chun-Miao;Xu, Zheng-Jun;Zhong, Wan-Fang

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关键词: intron-containing hairpin RNA;genomic DNA;recombinant-PCR;Zea mays;auxin-binding protein;DOUBLE-STRANDED-RNA;GENETIC INTERFERENCE;GENOMIC DNA;EXPRESSION

期刊名称:BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY ( 影响因子:2.043; 五年影响因子:2.017 )

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收录情况: SCI

摘要: Recently, much research on constitutive expression of an intron-containing self-complementary hair-pin RNA (ihpRNA) have been reported to silence target genes efficiently in a variety of species. Here we designed a new recombinant-PCR mediated method called direct amplification of ihpRNA from genomic DNA. This approach has proved to be easy, stable, and efficient.

分类号: Q

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