Infectious foot-and-mouth disease virus derived from a cloned full-length cDNA of OH/CHA/99

文献类型： 外文期刊

第一作者： Liu, GQ

作者： Liu, GQ;Liu, ZX;Xie, QG;Chen, YL;Bao, HF;Liu, XT

作者机构：

关键词： foot-and-mouth virus;OH/CHA/99;full-length cDNA;infectious clone;pBlFMDV

期刊名称：CHINESE SCIENCE BULLETIN （ 影响因子：1.649； 五年影响因子：1.738 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The China foot-and-mouth virus (FMDV) isolate OH/CHA/99 was isolated from swine, which was unable to infect bovine thyroid cells in vitro or to cause typical disease in bovines following intradermal inoculation in the tongue. To enhance antigenicity, replication, maturation and pathogenicity studies of OH/CHA/99, an infectious full-length cDNA clone, designated pBlFMDV, was prepared. The in vitro and in vivo biological properties of the virus derived from pBlFMDV were studied by analyzing antigenicity, plaque morphology and virulence in pigs. The results showed that the virus derived from pBlFMDV had the same biological properties as the parent strain OH/CHA/99; the full-length infectious cDNA clone, pBlFMDV, will be very useful in studies of the antigenicity, virulence, pathogenesis, maturation and replication of FMDV.

分类号： N1

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