The expression of SARS-CoV M gene in P Pastoris and the diagnostic utility of the expression product

文献类型： 外文期刊

第一作者： Han, XQ

作者： Han, XQ;Bartlam, M;Jin, YH;Liu, XT;He, XJ;Cai, XP;Xie, QQ;Rao, ZH

作者机构：

关键词： SARS coronavirus;recombinant M protein;antigenicity

期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.014； 五年影响因子：2.001 ）

ISSN： 0166-0934

年卷期： 2004 年 122 卷 1 期

页码：

收录情况： SCI

摘要： High-level protein expression is an important means of obtaining large amounts of viral proteins to investigate further their biological properties. To express the membrane (M) protein of SARS-CoV at high-level in vitro, the M gene fragment was amplified and cloned it into the Pichia Pastoris expression vector pPICZalphaA. SDS-PAGE and Western blotting analysis of the induced products of recombinant yeast transformant indicated that successful high-level expression of M protein was achieved, and that the expression product was similar antigenically to the natural protein. Purified recombinant M protein was used subsequently as an ELISA antigen for detection of eight serum samples screened previously by whole virus ELISA and immunofluorescence assay, and consistent results were obtained. These findings suggest that the recombinant M protein may be useful as a diagnostic reagent. (C) 2004 Elsevier B.V. All rights reserved.

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