Peptidoglycan recognition protein 6 (PGRP6) from Asian corn borer, Ostrinia furnacalis (Guenee) serve as a pattern recognition receptor in innate immune response

文献类型： 外文期刊

第一作者： Mei, Xianghan

作者： Mei, Xianghan;Peng, Peilin;Li, Chun;Qiao, Peitong;He, Enxi;Qiu, Zhiyong;Xia, Dingguo;Zhao, Qiaoling;Shen, Dongxu;Mei, Xianghan;Peng, Peilin;Li, Chun;Qiao, Peitong;He, Enxi;Qiu, Zhiyong;Xia, Dingguo;Zhao, Qiaoling;Shen, Dongxu

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关键词： agglutination; antimicrobial peptides; binding; Ostrinia furnacalis (Guenee); peptidoglycan recognition protein (PGRP)

期刊名称：ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：2.454； 五年影响因子：2.11 ）

ISSN： 0739-4462

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收录情况： SCI

摘要： Peptidoglycan recognition proteins (PGRPs) recognize invading microbes via detecting peptidoglycans from microbial cell walls. PGRPs are highly conserved from insects to vertebrates and all play roles during the immune defensive response. Ten putative PGRPs have been identified through transcriptome analysis in the Asian corn borer, Ostrinia furnacalis (Guenee). Whereas, the biochemical functions of most of them have not yet been elucidated. In this study, we found PGRP6 messenger RNA exhibited extremely high expression levels in the midgut, and its transcript level increased dramatically upon bacterial infection. Moreover, the enzyme-linked immunosorbent assay indicated recombinant PGRP6 exhibited a strong binding affinity to peptidoglycans from Micrococcus luteus and Bacillus subtilis, which could agglutinate M. luteus and yeast Pichia pastoris. Additionally, we demonstrated that PGRP6 was involved in the pathway of antimicrobial peptides synthesis, but could not enhance encapsulation and melanization of hemocytes. Overall, our results indicated that O. furnacalis PGRP6 serves as a pattern recognition receptor and detects peptidoglycans from microbes to initiate the immune response.

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