miR-212/132-Enriched Extracellular Vesicles Promote Differentiation of Induced Pluripotent Stem Cells Into Pancreatic Beta Cells

文献类型： 外文期刊

第一作者： Bai, Chunyu

作者： Bai, Chunyu;Gao, Yuhua;Bai, Chunyu;Guan, Weijun;Gao, Yuhua;Ren, Qiwei;Liu, Haifeng;Li, Xiangchen

作者机构：

关键词： iPSCs; beta cells; differentiation; extracellular vesicles; miRNAs

期刊名称：FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY （ 影响因子：6.684； 五年影响因子：7.219 ）

ISSN： 2296-634X

年卷期： 2021 年 9 卷

页码：

收录情况： SCI

摘要： Pancreatic beta cell transplantation is the ideal method for treatment of type 1 diabetes mellitus (T1DM), and the generation of beta cells from induced pluripotent stem cells (iPSCs) of patients is a promising strategy. In this study, we improved a previous strategy to produce beta cells using extracellular vesicles (EVs) derived from mature beta cells and differentiated beta cells from iPSCs (i-Beta cells), which secreted insulin under glucose stimulation in vitro and ameliorated hyperglycemia in vivo. Mechanistic analyses revealed that EV-carried microRNA (miR)-212/132 (EV-miR-212/132) directly bound to the 3 ' UTR of FBW7 to prevent its translation and FBW7 combined with NGN3 to accelerate its proteasomal degradation. EV-miR-212/132 stabilized NGN3 expression to promote differentiation of endocrine cells from induced iPSCs. Moreover, NGN3 bound to PDX1 to enhance transcription of endogenous miR-212/132 and formed a positive regulatory circuit that maintained the functions of mature pancreatic beta cells. Conclusion This study describes a novel approach for beta cell production and supports the use of iPSCs for cell replacement therapy of T1DM.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序